猫胃螺杆菌感染小鼠腺胃细胞动力学及其影响因素的研究  

Kinetic Changes of Gastric Epithelial Cells in H. Felis Infected Mouse and Its Affecting Factors

作　　者：曾志荣[1] 胡品津[1] 陈旻湖[1] 林丽莉[1] 陈为[1] 彭晓忠[1] 

机构地区：[1]中山医科大学附属第一医院消化内科,广州510080

出　　处：《华中医学杂志》1997年第3期101-103,共3页Central China Medical Journal

摘　　要：80只二级BALB/c小鼠随机分为二大组:实验组动物接种猫胃螺杆菌(HF)建立HF动物模型,感染HF16周后给予10％NaCl灌喂2次/周、25μg/ml N-甲基-N′-硝基-N-亚硝基胍(MNNG)自由饮用共12周,利用抗Brdu免疫组化、流式细胞计等技术比较各组的细胞动力学指标,结果实验组所有动物均感染HF,胃粘膜表现为慢性活动性胃炎;而对照组仅有极少数炎性细胞浸润。实验组各小组的标记指数LI、S期细胞比例S％、增殖指数PI均比对照组高(P<0.01)。Eighty two -grade BALB/c female mice were randomly divided into experimental group and control group. The mice in experimental group were inoculated H. felis(CS1) to establish HF mouse model. Sixteen weeks after H. felis infection, the mice were given 10% NaCl 2 times per week and 25μg/ml MNNG for 12 weeks. All mice were killed after 12 week-treatment. The kinetic changes of gastric epithelial cells were studied using anti - Brdu immunohistochemical staining and flow cytometry. The results showed that the cell kinetic indexes (LI.S% and PI) in experimental group were higher than those in the control group (P<0. 01). All mice in experimental group had H. felis infection with the stomach mucosa showing chronic active gastritis. However, only few inflammatory cells were found in stomach mucosa in control group.

关 键 词：胃肿瘤 胃螺杆菌 细胞动力学 动物实验 

分 类 号：R735.202[医药卫生—肿瘤]