rhEPO预处理对大鼠肝脏缺血再灌注损伤的作用  

Effect of rhEPO preconditioning on hepatic ischemia reperfusion injury in rats

作　　者：陈海云[1] 商中华[1] 钱惠岗[1] 张蓉婧[1] 

机构地区：[1]山西医科大学第二临床医学院普外科,太原030001

出　　处：《山西医科大学学报》2007年第11期981-984,1056,共5页Journal of Shanxi Medical University

摘　　要：目的探讨人重组促红细胞生成素(rhEPO)预处理对大鼠肝脏缺血再灌注损伤中的保护作用及其预处理较为合适的剂量。方法Wistar大鼠36只,随机分为对照组A、实验组B和C,每组12只。A组只给予夹闭肝蒂,B组和C组分别于缺血前30min皮下注射rhEPO5000U/kg和1000U/kg,在缺血45min及再灌注2h,取血清及肝组织行AST、ALT、TNF-α、IL-1β检测及HE染色、NF-κB的活化程度及计算肝细胞凋亡指数(AI)。结果与A组比较,B、C组肝细胞索排列较好,细胞坏死程度不明显。AST、ALT和TNF-α、IL-1β及NF-κB活化程度、AI在A组和B、C组之间均有显著差异(P<0.05),而在B、C组之间无统计学差异(P>0.05)。结论rhEPO对缺血再灌注损伤的肝脏具有保护作用,且以低剂量(1000U/kg)较为合适。Objective To explore the protective effect of rhEPO on hepatic ischemia reperfusion injury in rats and its suitable dosage. Methods Thirty-six Wistar rats were randomly divided into three groups： control group （group A, n = 12）, rhEPO preconditioning groups （groups B and C, n = 12 in each group）. Liver pedicles were occlused in group A. In groups B and C, the rats were subcutaneously injected rhEPO 5 000 U/kg and 1 000U/kg at 30 min before ischemia, respectively. After 45 min ischemia and 2 h reperfusion,the rats were killed. Serum aspartic acid aminotransferase（AST） and alanine aminotransferase （ALT）, tumor necrosis factor α（TNF-α） and interleukin 1β（IL-1β） were measured, and liver histopathology was observed by HE staining. Nuclear factor κB（NF-κB） was detected by immunohistochemistry, and apoptotic index was calculated by TUNEL. Results The parenchymal hepatic cell necrosis and structure derangement of hepatic lobules in groups B and C were lighter than in group A. Compared with group A, serum AST, ALT, TNF-α, IL-1β significantly decreased （P〈 0.05）, but there was no significant difference between groups B and C （ P 〉 0.05）. NF- κB was significantly higher in groups B and C than in group A（ P 〈 0.01 ）, and AI was significantly lower in groups B and C than in group A（P 〈 0.05）. Conclusion RhEPO could protect the liver from ischemia reperfusion injury in rats, and 1 000 U/kg is a suitable dose.

关 键 词：再灌注损伤 人重组促红细胞生成素 预处理 肝脏 

分 类 号：R364.12[医药卫生—病理学]