蒲黄中4个黄酮的对照品  

Chemical reference substance of four flavonoids in Pollen Typhae

作　　者：李维维[1] 徐伟[1] 关洪峰[1] 王冬[1] 刘建宇[1] 刘晓秋[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第11期703-705,709,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立蒲黄中对照品的研究方法。方法结合大孔吸附树脂、聚酰胺、Sephadex-LH20柱色谱方法对蒲黄中香蒲新苷(typhaneoside,1)、异鼠李素-3-O-新橙皮苷(isorhamnetin-3-O-neohes-peridoside,2)、柚皮素(naringenin,3)和异鼠李素(isorhamnetin,4)进行分离纯化,通过理化性质和光谱数据进行结构鉴定,TLC和HPLC进行纯度检查。结果制备得到的香蒲新苷、异鼠李素-3-O-新橙皮苷、柚皮素和异鼠李素的纯度分别为99.2%、99.7%、98.0%和96.2%。结论制备方法可作为蒲黄鉴别和含量测定用对照品。Objective To establish research method for the reference substances of Pollen Typhae. Methods Typhaneoside, isorhamnetin-3-O-neohesperidoside, naringenin and isorhamnetin were isolated and purified by macroporous resin, polyamide and Sephade x-LH20 column chromatography, and their structures were identified by physico-chemical properties and spectral data. Their purities were determined by TLC and HPLC. Results The purities of typhaneoside, isorhamnetin-3-O-neohesperidoside, naringenin and isorhamnetin were 99.2 %, 99.7 %, 98.0 % and 96.2 %, respectively. Conclusions This method is simple and effective to yield chemical constituents from Pollen Typhae, and these products can be used as reference substance for the research of Pollen Typhae.

关 键 词：蒲黄 对照品 制备工艺 结构鉴定 纯度检查 

分 类 号：R94[医药卫生—药剂学]