高效液相色谱法测定尿中咖啡因主要代谢物浓度  

HPLC determination of caffeine metabolites in urine

作　　者：易涛[1] 卢建丰[2] 

机构地区：[1]广州军区武汉总医院药局,武汉430070 [2]南京军区南京总医院药理科,南京210002

出　　处：《药学实践杂志》1997年第2期94-99,共6页Journal of Pharmaceutical Practice

摘　　要：本文建立了测定尿中咖啡因5种主要代谢物（AFMU、1X、1U、17X、17U）浓度的高效波相色谱法。尿样用硫酸铵饱和后加氯仿及异丙醇混合液（85：15）提取2次，空气流吹干，蒸馏水溶解进样。以ShimPackC18为固定相，甲醇-动腈-0．05％醋酸（10：1：89）为流动相，扑热息痛为内标，流速为1．2ml／min，在280um处定量检测。本法精确稳定可靠。用此方法测定了120名健康志愿者口服咖啡后的尿样，对人体内N-乙酰化转移酶和CYP1A2酶活性作了初步分析。A HPLC method was developed for determination of caffeine metabolites in urine(AFMU, 1X, 1U, 17X, 17U). The samplb added ammonium sulfate was extracted twice by a mix-ture of chloroform - isopropanol(85: 15 vol/vol)and evaporated to dryness with a gentle stream ofair below)25℃. HPLC analysis was carried out on Shim Pack C18 column with methanol - ace-tonitrile - 0. 05 % acetic acid (10: 1: 89 vol/vol) as the mobile phase and UV detection at 280nm.The assay procedure has been applied to determining urinary metabolites excreted after oral caf-feine in a healthy sample(n = 120). Caffeine metabolite ratios were used to assess the poymorphicN - acetyltransferase and CYP1A2 activities.

关 键 词：咖啡因 HPLC N-乙酰化转移酶 CYP_1A_2酶 

分 类 号：R971.7[医药卫生—药品] R927.2[医药卫生—药学]