口蹄疫病毒G-01株基因组编码区的序列分析  

Sequence Analysis of Genomic Coding Region of Foot-and-Mouth Disease Virus G-01

作　　者：索青利[1] 赵明秋[1] 陈金顶[1] 陈立军[1] 黄忠强[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《华南农业大学学报》2007年第4期95-99,共5页Journal of South China Agricultural University

基　　金：广东省自然科学基金(02099506025827);广东省科技计划项目(2005B20201006);广东省自然科学基金创新团队项目(5200638)

摘　　要：参考GenBank中注册的O型口蹄疫病毒（FMDV）全基因序列,分段设计了9对引物,应用RT-PCR方法对G-01株的基因组编码区序列进行了克隆和序列测定,获得了该毒株基因组编码区的核苷酸序列（6966 bp）并推导出其编码的氨基酸序列（2322 aa）.将获得的G-01株基因组编码区的核苷酸序列与参考的14株FMDV基因序列进行比较,结果表明,本毒株的3A蛋白的92～101位缺失10 aa,VP1基因的133～160位的关键位点没有变化,该毒株与以HKN/2002株为代表的7株O型毒株的同源性较高,与HKN/2002株遗传关系最近,而与另外7株O型毒株同源性较低,其遗传关系也较远.由此推断G-01与HKN/2002株属于遗传关系较近的毒株,这2株病毒有着相同的进化起源.The nucleotide sequence of the coding region of foot-mouth-disease virus（FMDV） strain G-01 was determinded via RT-PCR amplification,using 9 pairs of specific primers,which were designed according to the full-length genomic sequence of FMDV serotype O registered in the GenBank.Comparison of the nucleotide sequence and its deduced amino acid sequence with those of other 14 isolates revealed that G-01 was most closely related to 7 serotype O strains,and especially showed significant similarity with HKN/2002 found in Hong Kong.In contrast,the similarity of the comparable region in the other 7 strains was much lower,suggesting that they share less identity.Since G-01 appears to be most closely related to HKN/2002,it is proposed that these two strains share a common ancestor.

关 键 词：口蹄疫病毒 基因组编码区 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]