鼓室注射金黄色葡萄球菌致豚鼠鼓室硬化模型的建立  

Establishment of tympanosclerosis by intratympanic injection of Staphylococcosis aureus in guinea pigs

作　　者：李琰[1] 谢南屏[1] 万良财[1] 

机构地区：[1]南方医科大学珠江医院耳鼻咽喉科,广东广州510282

出　　处：《南方医科大学学报》2007年第11期1784-1786,共3页Journal of Southern Medical University

摘　　要：目的建立鼓室硬化的动物模型,探讨鼓室硬化发生过程。方法选择健康杂色豚鼠21只,麻醉后在无菌条件下于左耳经鼓膜注射1×10·8L-1金黄色葡萄球菌液100μl,制成慢性化脓性中耳炎模型,右耳为正常对照。术后1﹑3﹑6月分别断头处死动物,观察鼓膜和中耳粘膜的组织形态变化。结果在造模3月及更长时间的豚鼠实验耳中出现鼓室硬化病理改变,组织中钙质沉积及纤维化程度与慢性化脓性中耳炎持续的时间呈正相关。结论向豚鼠鼓室内注射金黄色葡萄球菌导致慢性化脓性中耳炎诱发鼓室硬化的动物模型切实可行。Objective To establish a guinea pig model of tympanosclerosis and investigate the development and progression of tympanosclerosis in the tympanic membranes and middle ear mocosa. Methods Twenty-one healthy guinea pigs were subjected inoculation of 1×10^8/L Staphylococcosis aureus into the left middle ear cavities under general anaesthesia, with the fight ears as the control, to establish models of chronic purulent otitis media. The animals were sacrificed by decapitation after 1, 3 and 6 months after model establishment for histological examination of the middle ear mocosa. Results The histological changes oftympanosclerosis occurred in some of the animals 3 months after model establishment, and the extent of calcium deposition and fibrosis across the mucosa were positively correlated with the duration of chronic purulent otitis media. Conclusion Tympanosclerosis may occur in some guinea pigs after inoculation of Staphylococcosis aureus into the middle ear cavity, and this model can be useful for study of tympanosclerosis.

关 键 词：鼓室硬化 豚鼠 中耳粘膜 金黄色葡萄球菌 

分 类 号：R-332[医药卫生]