葛根素对兔视网膜缺血/再灌注损伤的保护作用  

Protective Effects of Puerarin on Retinal Ischemic-Reperfusion Injury in Rabbits

作　　者：金敏[1] 朱远军[1] 高宗银[1] 杨为中[1] 胡燕飞[1] 郝绮红[1] 

机构地区：[1]广州医学院附属市一人民医院眼科,广东广州510180

出　　处：《广州医学院学报》2007年第3期14-16,共3页Academic Journal of Guangzhou Medical College

基　　金：广东省科技计划项目(2002C30903)

摘　　要：目的:动态观察葛根素对新西兰大白兔视网膜缺血-再灌注损伤的保护作用。方法:新西兰大白兔30只随机分为缺血再灌注对照组和葛根素治疗实验组每组15只,各组右眼应用前房灌注加压法使前房内压力升高至120 mmHg(1 mmHg=0.133 kPa)维持60 min后再灌注,再灌注后第12、24、72 h分别记录其视网膜电图,与缺血前视网膜电图相比,观察a、b波的变化。葛根素治疗组在造成缺血前2 h予葛根素溶液玻璃体腔内注射,对照组予以玻璃体腔内注射等体积生理盐水。结果:两组右眼在结束造模后立即进行视网膜电图记录所得图形均呈直线;对照组与实验组再灌注各时段视网膜电图a波振幅差异无统计学意义(P>0.05);实验组各时段b波振幅明显高于对照组(P<0.001)。结论:葛根素能促进缺血-再灌注损伤的视网膜功能恢复,对视网膜神经组织有保护作用。Objective：To observe the protective effects of Puerarin on ischemic-reperfusion injury in rabbit retina. Methods：Thirty New Zealand rabbits were randomized into the experiment group to receive puerarin or the ischemic- reperfusion group （ control group）, 15 in each. Retinal ischemia was induced in all rabbits by increasing intraocular pressure to 120mmHg for 60 minutes,followed by reperfusion. The ERG was used to measure the recovery of the a-wave and b-wave amplitudes for the experimental and control groups in different stages of reperfusion（ at 12,24 and 72 hrs）. Intravitreal puerarin was used in the experiment group 2 hours before retinal ischemia, whereas the control group received equivalent amount of normal saline. Results：The b-wave amplitudes in experimental group were much higher than those in the control group （ P 〈 0.01 ）. There were no significant differences in a-wave amplitude （ P 〉 0.05 ）. Conclusions.Puerarin has protective effects against ischemia reperfusion injury in rabbit retina.

关 键 词：视网膜 缺血再灌注损伤 葛根素 视网膜电图 

分 类 号：R774.1[医药卫生—眼科]