重症肌无力患者胸腺及外周血T淋巴细胞rDNA转录活性的研究  

Expression activity of T-lymphocyte rDNA in thymus and peripheral blood of myasthenia gravis patients

作　　者：全利国 姜学东[1] 张其刚[2] 王宪利[3] 芦伟[2] 孟庆显[1] 

机构地区：[1]武警辽宁总队医院胸心外科,沈阳110034 [2]中国医科大学附属第一医院胸外科,沈阳110001 [3]武警辽宁总队卫生处,沈阳110034

出　　处：《武警医学》2007年第11期816-817,共2页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨T淋巴细胞增殖活性在重症肌无力发病中的意义。方法应用银染技术对87例Ⅰ-Ⅱ期胸腺瘤合并MG和83例Ⅰ-Ⅱ期胸腺瘤不合并MG等自身免疫性疾病两组患者外周血和胸腺组织中T淋巴细胞进行细胞核嗜银染色,应用显微图像分析系统,计算核仁银染面积和核面积的比值做为核仁区嗜银蛋白(Ag-NORs)表达强度值,两组所测数据做t检验。结果胸腺瘤合并MG组外周血和胸腺组织中T淋巴细胞Ag-NORs表达强度均明显高于不合并MG组,两者差异有极显著统计学意义(P<0.001)。结论MG患者T淋巴细胞核蛋白体脱氧核糖核酸转录活性明显增强,细胞增殖活跃,进而导致体内兔疫功能亢进。Objective To study the proliferating activity of T-lymphocytes in myasthenia gravis patients. Methods Using argentaffin staining technique,the T-lymphocytes in thymus and peripheral blood of patients with thymoma in stages I and Ⅱ accompanied by myasthenia gravis（87 cases）and not by myasthenia gravis（83 cases）were stained and the area ratio of silver nitrate stained Ag- NORs to nucleus was calculated by computer micrography analysis. Student＇ s t-test was used to analyze the data. Results The Ag - NORs were expressed much higher in the myasthenia gravis group （ P 〈 0.001）. Conclusions The expression activity of T-lymphocyte rDNA in pafiemts with thymoma and concurrent myasthenia gravis is higher and the proliferation of cells is more active;thus the immune function is enhanced.

关 键 词：重症肌无力 核仁区嗜银蛋白 核蛋白体脱氧核糖核酸 

分 类 号：R736.3[医药卫生—肿瘤] R746.1[医药卫生—临床医学]