白首乌提取物对小鼠肝癌细胞H_(22)的抑制作用  

Anti-tumor efficacy of the extract from Radix Cynanchi Auriculati on mouse liver cancer Hepatoma 22

作　　者：毕芳[1] 陶文沂[1] 陆震鸣[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室

出　　处：《中成药》2007年第11期1586-1590,共5页Chinese Traditional Patent Medicine

摘　　要：目的:研究白首乌提取物对小鼠肝癌细胞H22的抑制作用。方法:建立H22细胞移植性肿瘤模型小鼠,随机分为白首乌低剂量(50μg/mL)组、中剂量(100μg/mL)组、高剂量(200μg/mL)组、空白对照组、模型组、阳性对照组共6组,给药15d后处死测量小鼠的瘤重,胸腺指数,脾指数和bax、bcl-2在瘤组织中的表达程度。结果:白首乌具有明显的抗肿瘤作用。结论:白首乌对小鼠肝癌细胞H22有抑制作用,对小鼠免疫器官无明显影响,同时,能够增加肿瘤组织中凋亡细胞的数量,增加抑癌基因bax蛋白的表达,并可减少癌基因bcl-2蛋白的表达。AIM： To study the anti-tumor efficacy of the extract trom the root of cynanchum aurwulatum Royie- ex Wight （RCA） on mice liver cancer Hepatoma 22. METHODS ： Hepatoma 22 tumor bearing mice models were established and then were divided randomly into six groups, including control group, Cynanchum auriculatum Royleex （high, middle and low dose） groups, model group, positive group. Each group included 10 mice. Mice were killed after medical treatment for 15 days and the tumor-weight-inhibiting rates .thymus index.spleen index and the degree of bax.bcl-2 in tumor were measured. RESULTS： The RCA showed significant anti-tumor action. CONCLUSION： The extract fro m the RCA shows anti-tumor efficacy on Hepatoma 22 tumor, and doesn＇ t show significant effect on thymus index.spleen index, increases the expression of bax and the quantity of apoptosis ceils of the tumor and decreases the expression of bcl-2.

关 键 词：白首乌 H22肝癌 免疫组化 抗肿瘤 

分 类 号：R285.6[医药卫生—中药学]