移植动脉新生内膜平滑肌细胞来源于受体骨髓细胞的实验依据  

Origin of smooth muscle cells in vascular neointima from recipient bone marrow cells in rat aortic allograft

作　　者：宋自芳[1] 李伟[2] 郑启昌[1] 管思明[2] 尚丹[1] 郭兴军[1] 

机构地区：[1]华中科技大学同济医学院协和医院普外科,湖北武汉430022 [2]华中科技大学同济医学院协和医院综合科,湖北武汉430022

出　　处：《中国病理生理杂志》2007年第11期2091-2095,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No30271242)

摘　　要：目的:研究大鼠移植动脉新生内膜平滑肌细胞Sry基因表达,探讨移植物动脉硬化新生内膜平滑肌细胞的来源。方法:将雄性Wistar大鼠骨髓细胞移植到雌性大鼠体内,制备嵌合体大鼠;4周后建立大鼠腹主动脉移植慢性排斥反应模型,分为4组:雌性同系移植组、雌性异系移植组、雄性异系移植组、嵌合体异系移植组。移植术后8周,取移植动脉组织标本,进行病理组织学观察与免疫组化染色,分析移植动脉新生内膜增生及新生内膜细胞α-SMA的表达;采用显微切割技术收集新生内膜平滑肌细胞,用PCR扩增方法检测细胞Sry基因表达,分析新生内膜平滑肌细胞与受体骨髓细胞的关系。结果:异系移植组移植动脉新生内膜中α-SMA表达阳性平滑肌细胞大量增生,致动脉内膜显著增厚,动脉壁新生内膜面积及新生内膜/中膜面积比均显著高于同系移植组(P<0.01)。PCR分析显示,嵌合体异系移植组和雄性异系移植组一致,在约225bp处均有1条特异性扩增条带,而雌性异系移植组则无相应的核酸扩增条带。结论:受体骨髓细胞作为移植物血管新生内膜平滑肌细胞的来源,参与移植物动脉硬化发生和发展。AIM： To study the expression of Sty gene in neointimal smooth muscle cells （ NI - SMCs）, and to investigate the origin of NI - SMCs in rat aortic allograft. METHODS： Sex - mismatched bone marrow transplantation was performed from male Wistar rat to female Wistar rat. Four weeks after transplantation, the aortic transplant model was constituted by means of micro - surgery in rat. The recipients were divided into four groups： female - female aortic isografts, female - female aortic allografts, male - male aortic allografts, female - chimera aortic allografts. Eight weeks after trans- plantation, aortic grafts were removed and processed for histological evaluation and immunohistochemistry assay. The Sty gene- specific PCR was performed on the genome of NI - SMCs to analyze its origin involved in aortic allograft. RESULTS ： Excessive accumulation of α- SMA - positive SMCs resulted in significant neointima formation in rat aortic allografts. The neointimal area and NIA/MA ratio of transplanted artery were significantly increased in all aortic allograft groups compared with those in aortic isograft group （ P 〈0.01 ）. PCR assay indicated that a distinct DNA band with 225 bp emerged in the male - male aortic allograft group and chimera aortic allograft group respectively, but not in the female - fe- male aortic allograft group, was observed. CONCLUSION ： As the origin of NI - SMCs, recipient bone marrow cells contribute to the pathological neointimal hyperplasia of aortic allograft.

关 键 词：动脉移植 新生内膜增生 平滑肌细胞 骨髓细胞 

分 类 号：R363[医药卫生—病理学]