Apelin在大鼠心肌缺血/再灌注损伤及缺血预处理中的作用  

Role of apelin in rat myocardial ischemic preconditioning and ischemia/reperfusion injury

作　　者：张明[1] 杨鹏麟[1] 童静[2] 龚永生[3] 范小芳[3] 唐疾飞[1] 金戈[1] 

机构地区：[1]温州医学院附属第二医院心内科,浙江温州325000 [2]温州医学院附属第二医院急诊科,浙江温州325000 [3]温州医学院机能实验教学中心,浙江温州325027

出　　处：《中国病理生理杂志》2007年第11期2112-2115,共4页Chinese Journal of Pathophysiology

基　　金：温州市科委基金资助项目(NoY2005A017)

摘　　要：目的:观察大鼠心肌缺血/再灌注损伤及预处理中新的小分子活性肽-apelin的变化,探讨其在该病理过程中可能的作用。方法:SD大鼠45只随机分成3组:缺血/再灌注(IR)组、预处理(IP)组和假手术(SH)组;心电图评估心律失常得分;放免法测定血浆及心肌apelin-36蛋白含量;免疫组化法观察心肌apelin-36的表达。结果:(1)心律失常得分:IP组(2.1±0.5)仅为IR组(3.6±0.8)的58.3%(P<0.01)。(2)血浆和心肌apelin-36浓度:IR组分别比SH组低36.1%和45.6%(均P<0.01);IP组比IR组分别高18.9%和38.5%(均P<0.05),但IP组仍分别比SH组低23.8%和24.7%(均P<0.01)。(3)免疫组化染色平均吸光度值:IR组比SH组低65.1%(P<0.01),IP组比IR组高80.8%(P<0.05),但比SH组低36.8%(P<0.05)。(4)心率失常得分与心肌组织中apelin-36蛋白含量呈显著负相关(r=0.847,P<0.01)。结论:在心肌缺血/再灌注损伤及预处理过程中,大鼠血浆及心肌组织apelin表达下调,提示apelin在该病理生理过程中可能有重要作用。AIM： To study the alteration and role of apelin in myocardial ischemic preconditioning and ischemia/reperfusion injury of rats. METHODS： Forty -five SD rats were randomly divided into three groups： ischemia/reper-fusion group （ IR）, ischemia pre - conditioning group （IP） and sham operation group. ECG was continuously used to eval- uate the score of arrhythmias. The protein levels of apelin - 36 in myocardium and plasma were detected by radioimmunoas- say. The expression of apelin was observed by immunohistochemistry. RESULTS： （1） The scores of arrhythmias in IP group （2.1±0.5） was only 58.3% of IR group （3.6±0.8） （P〈0.01）. （2） The apelin-36 protein level of plasma and myocardium in IR group were respectively lower by 36. 1% and 45.6% than those in SH group （P 〈0. 01 ）, and those in IP group were lower by 23. 8% and 24. 7% than those in SH group （ P 〈 0. 01 ）, but higher than those in IR group （ 18.9% and 38. 5%, respectively, P 〈0.05）. （3） The staining absorbance of apelin in IR, SH and IP group was （7. 87±2.41 ）, （22. 53±2. 54） and （ 14. 23±2. 15 ）, respectively. There were significant differences between IR and SH （ P 〈 0. 01 ） and between IP group and SH group （P 〈 0. 05 ）. （4） The scores of arrhythmias in IP and IR were negatively correlated with the protein level of apelin - 36 in myocardium （ r = 0. 847, P 〈 0. 01 ）. CONCLUSION ： The down - regu- lation of apelin - 36 in the plasma and myocardium of rats indicates that apelin has an important role in myocardial ischemic preconditioning and ischemia/reperfusion injury.

关 键 词：心肌再灌注损伤 APELIN 心律失常 大鼠 

分 类 号：R363[医药卫生—病理学]