持续阻断p44/42MAPK信号通路促进成骨前体细胞系MC3T3-E1分化  

Continuous blockage of the p44/42 MAPK pathway promotes the differentiation of MC3T3-E1 preosteoblasts

作　　者：马爱敏[1] 索伟[2] 邱力军[1] 漆家学[1] 

机构地区：[1]第四军医大学生物医学工程系,西安710032 [2]解放军总医院理疗科

出　　处：《陕西医学杂志》2007年第11期1472-1474,1491,共4页Shaanxi Medical Journal

摘　　要：目的:探讨p44/42MAPK信号通路对成骨前体细胞系MC3T3-E1分化的调控作用。方法:应用MEK1激酶的特异性抑制剂PD98059持续阻断p44/42MAPK信号通路在成骨前体细胞系MC3T3-E1中的活化;通过组织化学染色试验观察成骨前体细胞碱性磷酸酶活性以及体外钙质沉积情况;半定量RT-PCR试验检测成骨前体细胞特异性基因 mRNA的表达;荧光素酶分析试验测定成骨前体细胞特异性转录因子CBFA1基因启动子的活性。结果:PD98059不仅提高MC3T3-E1细胞的碱性磷酸酶活性,同时促进成骨前体细胞体外钙沉积和成骨特异基因的表达。而且PD98059作用后成骨特异性转录因子CBFA1启动子的活性也显著提高。结论:持续阻断p44/42MAPK信号通路促进成骨前体细胞分化。Objective： To study the regulatory effect of p44/42 MAPK pathway on the differentiation of MC3T3-E1 pre-osteoblast. Methods ： The activation of the p44/42 MAPK pathway was continuously suppressed with a specific MEK-1 inhibitor PD98059. The alkaline phosphatase activity and calcium deposition of MC3T3-E1 preosteoblasts were examined byhistochemicalstaining. The expressions of some osteoblast marker genes were evaluated by semiquantitative reverse transcription polynlerase chain reaction （RT-PCR）. The transcriptional activity of CBFA1 gene promoter was explored by Luciferase assay. Results ： PD98059 not only increased the Alkaline Phosphatase activity of MC3T3-E1 but also promoted calcium depostition and the expression of some osteoblast marker genes in MC3T3-E1 cells. The treatment of PD98059 significantly increased the Cbfal gene promoter activity. Conclusion ：Continuous suppression of the p44/42 MAPK pathway promotes the differentiation of MC3T3-E 1 pre-osteoblasts.

关 键 词：@p44/42MAPK 成骨细胞/免疫学 @MC3T3-E1 细胞分化 

分 类 号：R780.2[医药卫生—口腔医学]