不同疗程促红细胞生成素对大鼠脑缺血再灌注损伤后神经细胞凋亡的影响  

Effects of different erythropoietin treatment courses on apoptosis of neuronal cells in rats following ischemia-reperfusion injury

作　　者：丁正斌[1] 全伟[1] 曹志恺[1] 吕建平[1] 杨彬源[1] 张昊[1] 陈凡帆[1] 

机构地区：[1]广州医学院附属广州市第一人民医院神经外科,广州医学院2005级研究生510180

出　　处：《中华神经医学杂志》2007年第11期1122-1125,共4页Chinese Journal of Neuromedicine

基　　金：广州市医药卫生科技项目(2006-YB-029)

摘　　要：目的探讨不同疗程的促红细胞生成素(EPO)对局灶性脑缺血再灌注损伤大鼠神经细胞凋亡的影响。方法36只雄性SD大鼠随机分成EPO治疗组和生理盐水治疗组,每组分成短、中、长三个疗程,两组大鼠均制成局灶性脑缺血再灌注损伤模型。EPO治疗组短、中、长疗程相应时间点分别腹腔注射EPO 3000 U/kg;生理盐水治疗组各时间点注射等量生理盐水。疗程24 h后进行神经功能评分,断头取脑制作石蜡切片,免疫组化染色检测caspase-3,流式细胞仪检测神经细胞凋亡。结果与生理盐水组比较,EPO各疗程组神经功能相应地改善,而凋亡细胞与caspase-3表达均相应地减少,其中长疗程组减少最为显著,较中、短疗程有统计学差异(P<0.05)。结论不同疗程的EPO对缺血再灌注损伤后神经细胞凋亡的影响不完全相同,长疗程接近最佳疗程。Objective To explore the effects of different erythropoietin （EPO） treatment courses on apoptosis of neuronal cells in rats after ischemia-reperfusion injury （I-KI）. Methods A total of 36 male Sprague-Dawley （SD） rats were randomized into two groups： EPO treatment group and normal saline group. Each group was divided into three subgroups respectively with the short-, middle- and long-term therapeutic course and rat models of focal cerebral I-RI were established in both groups. EPO treatment groups received intrapcritoneal injection of EPO at 3 000 U/kg body weight respectively at a certain time point during the short-, middle- and long-term course of treatment, while the normal saline group received the same dose of normal saline. The neurological function of rats was scored at 24 h after administration; paraffin sections were produced with the removed rat brain decapitated at a certain time point of treatment; the expression of caspase-3 and apoptosis of neuronal cells were detected with immunohistochemical techniques and flow cytometry, respectively. Results The neurological function of EPO subgroups was relatively improved, compared with that of the normal saline group, but the number of apoptotic neuronal cells and expression of caspase-3 were all decreased, especially significantly in the long-term treatment group, which differed from that in the short- and middle term treatment groups significantly （/〈0.05）. Conclusion EPO of different courses of treatment exerts different impacts upon apoptosis of neuronal cells after I-KI and long-term EPO treatment course may be the optimal choice.

关 键 词：促红细胞生成素 脑缺血再灌注 细胞凋亡 CASPASE-3 

分 类 号：R741[医药卫生—神经病学与精神病学]