9-顺维甲酸联合细胞毒性药物对SH-SY5Y神经母细胞系增殖及诱导凋亡的影响  

Role of 9-cis Retinoic Acid plus Cytotoxic Drugs in the Proliferation and Apoptosis of SH-SY5Y Cell

作　　者：赵强[1] 史玉荣[1] 魏熙胤[1] 王景福[1] 张锦华[1] 

机构地区：[1]天津医科大学肿瘤医院天津市肿瘤防治重点实验室,天津市300060

出　　处：《中国肿瘤临床》2007年第21期1206-1208,共3页Chinese Journal of Clinical Oncology

基　　金：天津市教委科研基金资助(编号:20040217)

摘　　要：目的:探讨9-顺维甲酸联合细胞毒性药物对SH-SY5Y神经母细胞系增殖及诱导凋亡方面的作用,为9-顺式维甲酸治疗神经母细胞瘤可能有效方案的设计提供实验依据。方法:1)采用MTT比色法测定不同单药组和双药组在不同时间及不同浓度下SH-SY5Y细胞的抑制率;2)采用流式细胞仪测定不同单药组和双药组在不同时间及不同浓度下SH-SY5Y细胞的凋亡指数。结果:1)DDP+RA双药组中,低剂量组在24和48h以及高剂量组在48h细胞抑制率较相应DDP单药组高(P<0.01);UP-16+RA双药组中,低、高剂量组在24h细胞抑制率较相应UP-16单药组高(P<0.01);DDP+RA和UP-16+RA双药组(除了低剂量DDP+RA组在24h)细胞抑制率均高于RA组(500ng/孔)(P<0.01)。2)单药组和双药组细胞凋亡指数均高于对照组(P<0.01);双药组细胞凋亡指数均高于相应单药组(P<0.01)。结论:细胞毒性药物与RA组合用药细胞抑制率和早期细胞凋亡指数较单药高,提示细胞毒性药物与RA在抑制细胞生长和诱导凋亡方面表现很好的协同作用。Objective： To explore the role of 9-cis retinoic acid plus cytotoxic drugs in the proliferation and apoptosis of SH-SY5Y neuroblastic cell line. Methods： MTF assay was used to assess the cell growth inhibition in either mono-drug group and combined group. Flow cytometry was employed to detect the apoptosis index （AI） of neuroblastic cells. Results： DDP＋RA group＇s cell inhibition ratio of low dose at 24h and 48h and high dose at 48h was higher than that of the DDP group（P〈0.01）. UP-16＋ RA group＇s cell inhibition ratio of low dose and high dose at 24h was higher than that of the UP-16 group （P〈0.01）. Cell inhibition ratio of DDP＋RA group （except low dose group at 24h） and UP-16＋RA group was higher than that of RA group （P〈0.01）. AI of the mono-drug group and combined group was higher than that of the control group （P〈0.01）. AI of the combined group was higher than that of the mono-drug group（P〈0.01）. Conclusion： 9-cis retinoic acid and cytotoxic drugs may have a good synergistic effect on cell proliferation and apoptosis.

关 键 词：神经母细胞瘤 9-顺式维甲酸 增殖 凋亡 

分 类 号：R739.4[医药卫生—肿瘤]