神经节苷脂GM_3的分离纯化及对HL-60细胞膜中性糖脂图谱的影响  

ISOLATION OF GANGLIOSIDE GM 3 AND ITS INFLUENCE ON THE NEUTRAL GLYCOSPHINGOLIPID COMPOSITION OF HL 60 CELL MEMBRANE

作　　者：侯卫红[1,2] 张运晓[1,2] 张鹏 

机构地区：[1]河南医科大学 [2]河南濮阳县人民医院

出　　处：《白血病》1997年第1期10-12,共3页

摘　　要：用改进的Ｋｌｅｎｋ法从狗红细胞中提取和纯化了神经节苷脂ＧＭ３，将分离纯化的ＧＭ３加入早幼粒白血病细胞系（ＨＬ－６０）ＤＭＥ／Ｆ１２培养液中，终浓度为５０μＭ，细胞的增殖明显受到抑制，并按单核－巨噬细胞分化成熟，表现为细胞吞噬功能显著增强，非特异性酯酶活性升高。细胞的形态呈现与分化相一致的变化，比较ＧＭ３诱导ＨＬ－６０细胞分化前后细胞中性糖脂图谱的变化，ＣＤＨ显著增多是ＧＭ３诱导ＨＬ－６０细胞向单核－巨噬细胞分化的一个重要特征，ＣＭＨ及ＣＱＨ亦相应显著增多。The ganglionside GM 3 were extracted and purified from canine erythrocyte. We modified Klenks method slightly and obtained high purity GM 3. When a human promyelocytic leukemia cell line HL 60 cells were cultured with 50μM exogenous GM 3 in DEM/F12 medium supplemented with transferrin insulin SeO2 and 2% calf serum,cell growth was markedly inhibited. After 6 days of culture, HL 60 cells were induced to differentiate along the monocyte macrophage route. GM 3 treatment of HL 60 cells significantly increased the percentage of phagocytosing and NSE positive cell. On the basis of the observation, NGSL of undifferentiated cell and differentiated cells were separated and analysed by HPTLC. The results showed that after GM 3 treatment to induce monocytic macrophage differentiation, the most remarkable increase was CDH. Other NGSL such as CMH and CQH also showed significant increase.

关 键 词：神经节苷腺 HL-60细胞 中性糖脂图谱 

分 类 号：R73－3[医药卫生—肿瘤]