泻心汤有效组分不同配伍对内毒素肺损伤大鼠NF-κB及IκB表达的影响  

Effect of different compatibilities of effective fraction of Xie-Xin decoction on NF-κB and IκB expression of lung injury rats induced by LPS

作　　者：杨娜[1] 孟宪丽[1] 熊玉霞[1] 

机构地区：[1]成都中医药大学药理学教研室,四川成都610075

出　　处：《中国药理学通报》2007年第11期1441-1443,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30371756)

摘　　要：目的研究泻心汤有效组分及其配伍对内毒素肺损伤大鼠NF-κB及IκB表达的影响。方法大鼠灌胃给予黄芩总黄酮提取物(TFL)、大黄总游离蒽醌提取物(TFA)、大黄总结合蒽醌提取物(TCA)、TFL与TFA配伍高、低剂量(A高A低)、TFL与TCA配伍(B)及醋酸地塞米松(Dex)4d,末次给药后1h股静脉注射脂多糖(LPS)建立大鼠内毒素肺损伤模型,1、2、4h各组分别处死6只动物,收集肺组织,免疫印记(Western blot)检测核因子κB(NF-κB)、κB抑制因子(IκB)的蛋白表达。结果大黄总游离蒽醌、A高及Dex在1、2、4h均能够明显抑制NF-κB p65的核转位(P<0.01),所有给药组在2、4h均能够明显抑制胞质中的IκBα的降解(P<0.01)。结论泻心汤有效组分及其配伍对内毒素肺损伤大鼠保护作用与抑制NF-κB的核转位及IκB的降解有关。Aim To investigate the effect of effective fraction of Xie-Xin decoction and their compatibilities（EFX）on NF-κB and IκB expression of lung injury rats induced by LPS.Methods Rats were pretreated for 4 days with total flavones（TFL）,total free anthraquinones（TFA）,total conjugated anthraquinones（TCA）,the high（low）dose of compatibility of TFL and TCA（A high,A low）,the compatibility of TFL and TCA（B）and dexamethasone respectively 4 days,then injected intravenously with LPS to induce lung injury.6 rats were sacrificed respectively in every group at 1,2,4 h after LPS administration,and the right middle of the lung was harvested at every time point,the expression of NF-κB and IκB was evaluated by Western blot.Results TFA、A high and Dex can inhibit the nuclear translocation of NF-κB at 1,2,4 h（P〈0.01）,and all the groups can inhibit the degradation of IκBα at 2、4 h（P〈0.01）.Conclusion The protection of EFX on LPS-induced lung injury rats is related to the inhibition of NF-κB nuclear translocation and IκB degradation.

关 键 词：内毒素 泻心汤 肺损伤 NF-ΚB IΚB 

分 类 号：R-332[医药卫生] R289.1