蛇床子素对动物体内雌二醇和脂酶的影响及其相互关系的研究  

Study of the effects of osthole on blood estradioland lipase in animals and their relationship

作　　者：张岩[1] 谢涛[2] 薛洁[1] 朱路佳[1] 华轶男[1] 谢梅林[1] 顾振纶[1] 

机构地区：[1]苏州大学医学院药理学教研室 [2]滨州医学院临床医学系,山东滨州264003

出　　处：《中国药理学通报》2007年第11期1476-1480,共5页Chinese Pharmacological Bulletin

基　　金：苏州市"三药"科技攻关基金资助项目(NoSSY0608)

摘　　要：目的观察蛇床子素对体内血脂、雌二醇(E2)和脂酶的影响及其相互关系。方法在建立高脂血症小鼠模型的同时,分组给予蛇床子素10～40mg.kg-1,3wk后测定肝素化后血浆LPL和HL活性及血浆TC、TG和HDL-C水平。分组给予正常小鼠蛇床子素10～40mg.kg-1,1wk后用ELISA方法观测血清E2水平的变化。建立去卵巢大鼠模型,给予蛇床子素10mg.kg-1,12wk后测定血清E2、TC和TG水平。结果给予蛇床子素10～20mg.kg-1后,能升高高脂血症小鼠肝素化后血浆LPL、HL活性和正常小鼠血清E2水平(P<0.05或P<0.01),也可使高脂血症小鼠血浆TC、TG和LDL-C降低(P<0.05或P<0.01),升高HDL-C(P<0.01)。蛇床子素剂量增至40mg.kg-1时,虽能升高正常小鼠血清E2水平(P<0.01),但高脂血症小鼠的血浆LPL、HL活性反而降低,对降低血浆TC、TG、LDL-C和升高HDL-C水平的幅度也不如低剂量的蛇床子素。去卵巢大鼠给予蛇床子素10mg.kg-1后,能增加血清E2水平(P<0.05),也能降低血清TG水平(P<0.05),TC的水平也有一定的降低。结论较低剂量的蛇床子素可通过增加血中E2水平,提高LPL和HL活性而降低血脂水平,较高剂量的蛇床子素虽可进一步增加血中E2水平,但LPL和HL活性反而降低,导致调脂作用减弱。Aim To study the effects of osthole on blood lipid,estradiol（E2）,lipoprotein lipase（LPL）,hepatic lipase（HL）and their relationship.Methods Mice were orally given osthole 10~40 mg·kg^-1 when hyperlipidemic model was induced.After 3 weeks,activities of LPL and HL and total cholesterol（TC）,triglyceride（TG）and high density lipoprotein-cholesterol（HDL-C）levels in post-heparin plasma were determined.Normal mice were treated with osthole 10~40 mg·kg^-1 for 1 week,the serum level of E2 was determined by an ELISA method.Ovariectomized rats were orally given osthole 10 mg·kg^-1 for 12 weeks,then serum E2,TC and TG levels were measured.Results After treatment with osthole（10~20 mg·kg^-1）,post-heparin plasma activities of LPL and HL in high-fat diet mice and serum level of E2 in normal mice significantly increased（P〈0.05 or P〈0.01）,plasma lev-els of TC,TG and low density lipoprotein-cholesterol（LDL-C）in high-fat diet mice decreased（P〈0.05 or P〈0.01）,and HDL-C level increased（P〈0.01）.When the dose of osthole increased to 40 mg·kg^-1,the serum level of E2 further increased（P〈0.01）,but activities of LPL and HL in post-heparin plasma in high-fat diet mice decreased,and the degree of decrease in plasma levels of TC,TG,LDL-C and increase in HDL-C level were also lowered as compared with low-dose osthole.After ovariectomized rats were treated with osthole 10 mg·kg^-1 for 12 weeks,serum E2 obviously increased（P〈0.05）,while TG level significantly decreased（P〈0.05）,and serum level of TC also decreased in some degree.Conclusions Low-dose osthole could increase the blood level of E2 and the activities of LPL and HL,and result in decrease in the blood lipid.Though high-dose osthole could further increase the E2 level in blood,the activities of LPL and HL decreased and its lipid-modifying effect was attenuated as well.

关 键 词：蛇床子素 雌二醇 脂蛋白脂酶 肝脂酶 

分 类 号：R-332[医药卫生] R282.71