王浆高产蜜蜂与意大利蜜蜂卵期蛋白质组比较  

Comparative Analysis of the Proteome of the Egg of the Higher Royal Jelly Producing Bees (Apis mellifera L.) and Italian Bees (Apis mellifera L.)

作　　者：李建科[1] 张兰[1] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093

出　　处：《中国农业科学》2007年第11期2632-2636,共5页Scientia Agricultura Sinica

基　　金："十一五"国家科技支撑计划重点项目(2006BAD06B04;2006BAD12B08-06);中央级公益性科研院所基本科研业务费专项(JBKY0032007016CAAS);公益性行业科研专项(nyhyzx07-041)资助

摘　　要：【目的】比较王浆高产蜜蜂(Apis mellifera L.浆蜂)与原种意大利蜜蜂(Apis mellifera L.原种意大利蜜蜂)工蜂卵期3d发育阶段蛋白质组。【方法】采用双向电泳的方法比较两个蜂种卵期发育蛋白质组。【结果】结果显示,两个蜂种卵期3d所得6张胶图检测到的蛋白点均具有相同的分子量与等电点范围,分子量范围为11.00～94.00kD,等电点范围为3.40～8.60。在工蜂卵3d的发育过程中,浆蜂分别检测到502、523和516个蛋白点,而原种意大利蜜蜂分别检测到349、361和354个蛋白点。同时,卵期3天发育过程中,两个蜂种分别检测到180、151和197个共有的表达蛋白。此外,浆蜂还检测到322、372和319个特有蛋白,而原种意大利蜜蜂也分别检测到169、210、157个特有蛋白,这些蛋白90%以上都是低表达量蛋白。【结论】在工蜂胚胎发育过程中,浆蜂有更多的基因参与表达调控,特有蛋白可能与调节王浆产量相关的基因相关。[Objective] The purpose of the study was to investigate and compare the protein composition of the higher producing royal jelly bees （Apis mellifera L.） and the native Italian bees （Apis mellifera L.） during three days egg development of the worker bees. [Method] Two-dimensional gel electrophoresis （2-DE） employed to separate and quantify proteins of the worker bees＇ egg development of the two bee species. [Result] The results showed that all the expressed protein spots on the six gels with the same range of molecular weight （MW） and pI in terms of 11.00-94.00 kD and 3.40-8.60, respectively. The detected spot number on day 1, day 2 and day 3 of the higher production royal jelly worker bees were 502, 523 and 516, respectively. Whereas, the detected spot number on day l, day 2 and day 3 of the unselected native Italian bees were 349, 361,354, respectively. Meanwhile, there were 180, 151, 197 mutual-matched proteins between the two bee species corresponding to the eggs＇ 3 days development. Furthermore, 322, 372, 319 specific proteins in the higher production royal jelly bees and 169, 210, 157 specific proteins in the unselected native Italian bees, 90 percent of low abundance in both of them. [Conclusion] The results indicate that significantly higher numbers of genes are expressed in the royal jelly producing bees than those of the Italian bee during their embrogenesis. And the specific proteins are probably concern with royal jelly production.

关 键 词：蜜蜂 王浆 工蜂卵 双向电泳 蛋白质组 

分 类 号：S891[农业科学—特种经济动物饲养]