突变的大肠癌mtDNA影响NIH3T3细胞增殖和凋亡的研究  

The influence of mutated mtDAN of colorectal carcinoma on the growth and apoptosis of NIH3T3 cell line

作　　者：姬宏莉[1] 肖冰[1] 陈佳奇[1] 张宇声[1] 宋卫兵[1] 姬宏娟[2] 

机构地区：[1]南方医科大学南方医院消化病研究所 [2]中国人民解放军71511部队卫训队

出　　处：《广东医学》2007年第11期1730-1732,共3页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:037049)

摘　　要：目的初步研究突变的大肠癌mtDNA与肿瘤发病机制的关系。方法将已构建的重组表达载体转染NIH3T3细胞,通过MTT法及流式细胞仪(FCM)分别观察其增殖和凋亡的影响。结果各组间NIH3T3细胞的凋亡率不同,差异有显著性(P<0.001),各组间NIH3T3细胞的增殖差异有显著性。结论突变的大肠癌mtD-NA可通过影响NIH3T3细胞的生物学行为,致其有恶性转化倾向,但具体的机制和过程有待于进一步研究。Objective To study the relationship between mutation mtDAN and tumor pathogenesis. Methods After transfection of the recombinaut expressing vector into NIH3T3 cells line, the changes in the growth and apoptosis in different groups were examined by MTT and flow cytometry. Results The apoptosis and growth rate were significantly dif- ferent in different groups. Conclusion The mutated mtDNA may affect the biological behaviors, leading to a tendeny of malignant tranformation. However, the mechanism and process of tumorogenesis need further investigation.

关 键 词：线粒体DNA 细胞核基因组 转染 

分 类 号：R730.231[医药卫生—肿瘤]