改良蛋氨酸胆碱缺乏饮食喂养的非酒精性脂肪性肝炎大鼠模型的建立  

Establishment of nonalcoholic steatohepatitis rat models with modified methionine-choline-deficient diets

作　　者：钱晓武[1] 范竹萍[2] 汪晓红[2] 盛黎[2] 奚志峰[2] 邱德凯[2] 

机构地区：[1]泰州市人民医院老年科,江苏省泰州市225300 [2]上海交通大学医学院附属仁济医院上海消化疾病研究所,上海市200001

出　　处：《世界华人消化杂志》2007年第28期2983-2989,共7页World Chinese Journal of Digestology

摘　　要：目的:观察改良蛋氨酸胆碱缺乏饮食(MCD)建立的脂肪性肝炎大鼠模型肝脏的脂肪变性和炎症情况.方法:大鼠26只随机分为4组,分别喂养MCD饮食和胆碱添加饮食(CS).3-8wk后处死,肝脏标本在甲醛中固定和石蜡包埋.HE染色和Masson染色后对脂肪变性、炎症和纤维化进行评分.血清ALT,AST,CH,LDL等指标通过生化分析仪进行测定.结果:MCD喂养组3wk可以看见肝脂肪变性和炎症,肝指数显著增加,ALT和AST显著升高,8wk可见纤维形成.结论:可通过改良的蛋氨酸胆碱缺乏(MCD)饮食建立非酒精性脂肪肝动物模型.AIM： To establish nonalcoholic steatohepatitis （NASH） rat models with modified methioninecholine-deficient （MCD） diets. Hepatic steatosis and inflammation would be expected in these rats. METHODS： Rats were randomly divided into 4 groups. Rats were fed with MCD or with cholinesupplemented （CS） diets, Weight and food intake were determined. After 3 and 8 weeks, rats were sacrificed; liver specimens were fixed overnight in buffered formaldehyde （40 g/L） and embedded in paraffin, HE-stained and Massion-stained sections were graded blindly for the degree of fatty change, inflammation, fibrosis and necrosis, Serum was measured for biochemical markers, RESULTS： After 3 weeks on an MCD diet, steatosis and inflammation could be seen in rat livers. The liver index was increased significantly by the MCD diet. Serum ALT and AST levels were also increased significantly, whereas TG levels decreased. After 8 weeks on the diet, fibrogenesis could be seen. CONCLUSION： Rat NASH models can be established using modified MCD diets.

关 键 词：蛋氨酸胆碱缺乏饮食 非酒精性脂肪性肝炎 脂肪变性 

分 类 号：R575.5[医药卫生—消化系统]