成牙本质细胞中上游刺激因子1对骨桥素表达调控的研究  

Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1

作　　者：吴礼安[1] 文玲英[1] 杨富生[1] 王小竞[1] 

机构地区：[1]第四军医大学口腔医学院儿童口腔科,西安710032

出　　处：《中华口腔医学杂志》2007年第11期663-664,共2页Chinese Journal of Stomatology

摘　　要：目的检测成牙本质细胞中上游刺激因子1(upstream stimulatory factor 1,USF1)对骨桥素表达的影响。方法培养成牙本质细胞 MDPC-23,稳定转染 PCMV-USF1和酸性-USF(A-USF)质粒,提取总 RNA,半定量反转录聚合酶链反应检测骨桥素的表达水平,并对结果进行统计学分析。结果筛选出稳定转染 USF1和 A-USF 的抗性克隆,USF1、A-USF 转染组和对照组骨桥素相对灰度比值分别为:60.33%±4.51%、229.33%±7.09%、110.00%±15.62%,转染组与对照组差异有统计学意义(P<0.01)。结论 USF1可调控成牙本质细胞骨桥素 mRNA 转录,该作用被 A-USF 部分阻断。Objective To investigate the regulation effects of upstream stimulatory factor 1 （USF1） on osteopontin expression in edontoblasts. Methods Odontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed. Results Clones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group. Conclusions Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF.

关 键 词：牙本质生成 逆转录聚合酶链反应 成牙本质细胞 

分 类 号：R78[医药卫生—口腔医学]