黏膜相关淋巴组织结外边缘区B细胞淋巴瘤及其转化细胞的克隆性  

Clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue lymphoma

作　　者：江炜[1] 李甘地[1] 李雷[1] 唐源[1] 何艳梅[1] 

机构地区：[1]四川大学华西医院病理科,成都610041

出　　处：《中华病理学杂志》2007年第11期736-741,共6页Chinese Journal of Pathology

基　　金：国家自然科学基金(30370600)

摘　　要：目的分析黏膜相关淋巴组织结外边缘区 B 细胞淋巴瘤(MALT 淋巴瘤)中转化与未转化肿瘤细胞间的克隆联系。方法选具有大细胞转化的、可用于激光显微切割的 MALT 淋巴瘤6例为研究对象,采用 EliVision^(TM)方法检测 bcl-10等蛋白表达,逆转录聚合酶链反应(RT-PCR)检测API2-MALT1融合基因,激光显微切割进行目的细胞群分离,PCR 检测免疫球蛋白重链(IgH)基因重排并对产物进行测序分析。结果伴有转化的6例中1例胞质和胞核同时表达 bcl-10蛋白,2例检测出 API2-MALT1融合基因。同一例不同肿瘤细胞群分别显示同样的基因重排序列。例2和例5在N区和 D 区有两个核苷酸位点的碱基不同。结论伴有转化的6例 MALT 淋巴瘤转化和未转化肿瘤细胞群均分别来自同一个肿瘤克隆。Objective To analyze the clonal relationship between transformed and non-transformed components in mucosa-associated lymphoid tissue （MALT） lymphoma. Methods Six cases of MALT lymphoma with high-grade transformation were studied. Immunohistochemical study was carried out by EliVision using bcl-10 antibody. Reverse transcription-polymerase chain reaction was used to detect the presence of API2-MALT1 fusion gene transcripts. The target cells were selected by laser microdissection and studied by polymerase chain reaction and sequence analysis for rearrangement of immunoglobulin heavy chain gene. Results In the 6 cases of MALT lymphoma with high-grade transformation, nuclear and cytoplasmic expression of bcl-10 was demonstrated in 1 case, while API2-MALT1 fusion gene was present in 2 cases. Identical fragments of rearranged immunoglobulin heavy chain gene were detected in both transformed and non-transformed components in each of the 6 cases, except for the differences at 2 nucleotide positions in N or D regions in 2 cases. Conclusion The tumor cells from both transformed and non-transformed components in MALT lymphoma derive from the same clone.

关 键 词：淋巴瘤 黏膜相关淋巴样组织 克隆 分子 显微切割 蛋白质bel-10 

分 类 号：R733.1[医药卫生—肿瘤]