高活力液体碱性蛋白酶制备工艺研究  

Study on pilot preparation technology for high activity and alkaline liquid protease

作　　者：孙同毅[1] 李军训[1] 殷向彬[1] 陈坤[1] 路福平[1] 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300457

出　　处：《现代化工》2007年第11期31-33,共3页Modern Chemical Industry

基　　金：国家高技术研究发展计划("863"计划)(2007AA02Z212);国家科技资源平台项目(2005DKA21204-10);科技部中小企业创新基金(06C26211200692)

摘　　要：为了获得高质量浓缩液体蛋白酶,对提取工艺中不同絮凝剂,不同大小活性炭以及膜过滤条件进行了优化。结果表明0.1%质量分数NaAlO2絮凝剂在pH=8.0时絮凝速度快、效果好;1%中型颗粒活性炭脱色效果明显;聚丙烯膜超滤温度10℃、压力0.05 MPa时酶的回收率最高。优化后的工艺使液体酶活力提高到3.2×105μ/mL,使初始发酵液浓缩了8.3倍,酶回收率达到83.6%。Optimization of flocculent agents, active carbon and conditions of filter membranes are studied in the pilot preparation technology to obtain high quality liquid protease. The results show that 0.1% of NaAlO2 is the best in pH 8.0 for it, and 1% of carbon medium-sized active carbon the best too. The conditions of treating temperaure of 10℃ and operating pressure of 0.05 MPa is optimal for ultrafiltration concentration with polypropylene filter membranes. Under these conditions, a 3.2 ×10^5μ/mL of liquid protease can be produced. And the enzyme can be condensed by 8.3 times from broth, with a recovery rate of 83.6%.

关 键 词：液体蛋白酶 制备工艺 絮凝 超滤浓缩 

分 类 号：TQ426.97[化学工程]