脱钙牙纳米材料的制备与细胞毒性研究  

Preparation and cytotoxic evaluation of decalcified dentin matrix nanomaterials.

作　　者：王虎中[1] 乔学亮[2] 王翔[1] 郭家平[1] 石咏梅[1] 唐瑛[1] 王早堂[1] 

机构地区：[1]广州军区武汉总医院口腔科,湖北武汉430070 [2]华中科技大学材料学院

出　　处：《临床口腔医学杂志》2007年第11期646-649,共4页Journal of Clinical Stomatology

摘　　要：目的:分别以玛瑙球和不锈钢球为球磨介质球磨法成功制备脱钙牙纳米材料,通过体外细胞毒性试验,对两种不同球磨介质制备的脱钙牙纳米材料的生物相容性进行了研究。方法:选用不同体积分数常温下的脱钙牙纳米材料浸提液为培养液,检测1d、3d、5d小鼠成纤维细胞L929的光吸收值,计算相对增殖率,确定脱钙牙纳米材料的毒性程度。结果:两种方法制备的脱钙牙纳米材料的细胞毒性为0级,有着良好的细胞生物相容性。以玛瑙为球磨介质制备的脱钙牙纳米材料的细胞相对增殖率大于同种条件下以不锈钢球为球磨介质制备的脱钙牙纳米材料的细胞相对增殖率。结论:以玛瑙为球磨介质制备的脱钙牙纳米材料较以不锈钢球为球磨介质制备的脱钙牙纳米材料具有较高的细胞相对增殖率。Objective：Two kind of decalcified dentin matrix （DDM） nanomaterials were successfully prepared by a ball milling process with agate ball and stainless steel ball as ball milling media. The biocompatibility of the nanomaterials was investigated through in vitro Cytotoxic experiments. Method： In this study, different concentrations of decalcified dentin nanomaterials leaches were prepared under normal temperature. In order to determine the effects of DDM on the cell growth, Fibroblast Cells of mice L929 were cultivated directly in the animal culture media （RPMI1640） containing different concentrations of DDM leaches at 37 ~C and 5% CO2 for one, three and five days. The optical density （OD） of L929 cells was then measured and the relative growth rate （RGR） of cells was calculated, based on the results of MTF assay.Result： Our experimental results show that the toxicity grades of DDM proves all zero, with good biocompatibility in vitro. It was also found that the relative growth rate of cells for agate ball as milling media is higher than that of stainless steel ball as milling media, which maybe results from the impurity imported during the milling process with stainless steel ball as milling media. Conchusion： the relative growth rate of cells for agate ball as milling media is higher than that of stainless steel ball as milling media.

关 键 词：脱钙牙基质 纳米材料 细胞毒性 MTT 

分 类 号：R783.1[医药卫生—口腔医学]