16SrDNA分型技术在单核细胞增生李斯特菌分型中的应用  

Application of 16 SrDNA molecular typing to produce dendrogram of L.monocytogenes

作　　者：梅玲玲[1] 朱敏[1] 潘雪霞[1] 张俊彦[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2007年第11期1963-1964,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立浙江省食品中单核细胞增生李斯特菌系统树,为食物中毒事件追源提供依据。方法：采用PCR扩增16SrDNA的方法,对浙江省2000～2005年从食品中分离的36株单核细胞增生李斯特菌进行分子分型,并结合MEGA软件构建单核细胞增生李斯特菌带型关系系统树状图。结果：所采用PCR方法可使单核细胞增生李斯特菌扩增出1.5 kb大小的条带,应用MEGA软件构建的系统树可以将36株单核细胞增生李斯特菌划分为两大类,18个分支;单核细胞增生李斯特菌血清型与16SR rDNA系统树无关联。结论：16SrDNA分型方法可以对单核细胞增生李斯特菌进行有效分型,可为单核细胞增生李斯特菌食源性疾病的流行病学调查和溯源提供科学依据。Objectlve：To produce the dendrogram of L. monocytogenes in the food of Zhejiang Province so as to provide evidence to trace food toxic case. Methods：PCR was utilized in amplification of 16srDNA to conduct molecular typing of 36 strains of L. monocytogenes isolated from food samples in Zhejiang Province from year 2000 to 2005. MEGA software was employed to produce the dendrogram. Result： 1.5 kb DNA band was amplified from L. monocytogenes by adapting PCR, and the 36 isolated stains of L. monocytogenes were divided into two categories, 18 branches, according to the dendrogram established by employing MEGA software. The blood serum of L. monocytogenes was not related to the 16SRrDNA dendrogram. Conclusion： 16SRrDNA molecular typing method can be used in the effective classification of L. monocytogenes to provide scientific support to the epidemiological investigation and source - tracking of food - caused diseases by L. monocytogenes.

关 键 词：单核细胞增生李斯特菌 16SrDNA 分子分型 

分 类 号：R155.5[医药卫生—营养与食品卫生学]