肝靶向药物Gal-HSA-5-FU对Bel-7402肝癌细胞增殖和细胞周期的影响  

Effect of hepatic targeting drug Gal-HSA-5-FU on proliferation and cell cycle of human hepatocarcinoma cells(Bel-7402)

作　　者：李育超[1] 周克元[1] 蔡春[2] 张海涛[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所 [2]广东医学院分析中心,广东湛江524023

出　　处：《广东医学院学报》2007年第5期515-518,共4页Journal of Guangdong Medical College

摘　　要：目的了解合成的肝靶向药物半乳糖化人血清白蛋白与5-氟尿嘧啶偶联物（galactosylated human serum albumin 5-fluorouracil conjugate，Gal-HSA-5-FU）对肝癌细胞Bel-7402细胞增殖和细胞周期的影响。方法采用MTT法（噻唑蓝还原法）检测Gal-HSA-5-FU对细胞生长的抑制率和IC50，流式细胞术分析细胞周期变化，荧光染色法观察细胞凋亡。结果0．953-30．500mg·L^-1 Gal-HSA-5-FU分别处理Bel-7402细胞24、48、72h，细胞增殖被抑制，其增殖抑制率随药物质量浓度的增加和作用时间的延长而增高，其IC50值分别为（20．957±0．752）、（11．793±0．546）、（5．843±0．735）mg·L^-1，48、72h的IC50值明显低于24h的IC50值，差异均有统计学意义（P〈0．01）。相同质量浓度5-FU与Gal-HSA-5-FU作用24h，其IC50值差异无统计学意义（P〉0．05）。Gal-HSA-5-Fu作用Bel-7402 24h，S期细胞明显升高，G2／M期细胞明显下降。荧光染色偶见凋亡细胞。结论Gal-HSA-5-FU能抑制Bel-7402细胞增殖，且抑制作用具有剂量和时间依赖性；一定剂量的Gal-HSA-5-FU可能通过阻滞Bel-7402于S期而抑制其增殖。Objective To investigate the effect of Galactosylated human serum albumin 5-fluorouracil conjugate （Gal-HSA- 5-FU） on proliferation and cell cycle in human hepatocarcinoma cells （Bel-7402）. Methods The inhibitory rates of cell proliferation and IC50 values of Gal-HSA-5-FU were determined by MTT assay. Cell cycle of the tumor cells was analyzed with flow cytometry. Results Cell proliferation was inhibited after the cells were treated with various concentrations of Gal-HSA-5-FU （ranging from 0. 953-30. 500mg·L^-1 ） for 24, 48, and 72h, respectivdy. The inhibitory rates of cell proliferation were enhancing with the increase of drug concentration and treatment time （ P〈0.01 ）. The IC50 values of Gal-HSA-5-FU were （20. 957 ± 0. 752 ）, （ 11. 793 ± 0. 546 ）, and （ 5. 843 ± 0. 735 ） mg·L^-1 at 24, 48 and 72h, respectively. The IC50 values at 48h and 72h were significantly lower than that at 24h （ P〈 0.01 ）. After the treatment of 24h, the proportion of Bel-7402 cells in the S phase increased, while the proportion in the G2/M phase decreased. There was little number of apoptotic cells according to the fluorescence staining assay. Conclusion Gal-HSA-5-FU can inhibit the proliferation of Bel-7402 cells in a dose- and time-dependent manner, and can arrest Bel-7402 cells in S phase.

关 键 词：Gal-HSA-5-FU 肝癌细胞 增殖 细胞周期 

分 类 号：R730.53[医药卫生—肿瘤] R735.7[医药卫生—临床医学]