HPV和VEGF及ER在子宫颈癌及其癌前病变中的表达研究  

A study on the expression of HPV,VEGF and ER in the cervical carcinoma and its precancerous lesions

作　　者：姜星[1] 曾子华[2] 周序珑 王庆一[1] 

机构地区：[1]广东省珠海市妇幼保健院妇科,广东珠海519000 [2]暨南大学医学院病理教研室,广州510630

出　　处：《中国肿瘤临床与康复》2007年第5期389-392,共4页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨HPV、VEGF和ER在慢性宫颈炎、宫颈上皮内瘤变（CIN）及浸润性宫颈癌（ICC）中的表达，以及它们之间的相互关系，旨在提高宫颈上皮内瘤变及浸润性宫颈癌的诊断率。方法采用原位杂交（HC11）方法检测40例慢性宫颈炎、CINⅠ～Ⅱ组30例、CINⅢ组25例以及ICC组30例中高危HPV的表达，并采用免疫组化S—P法检测上述病例中VEGF和ER的表达。结果慢性宫颈炎、CINⅠ～Ⅱ组织很少表达HPV，显著低于CINIU组织和ICC，差异有显著性（P〈0．05）；慢性宫颈炎组织、CINⅠ～Ⅱ组织中很少表达VEGF，但是在CINIU组及ICC组织中呈高表达，二者之间差异有显著性（P〈0．05）；ER在慢性宫颈炎组织中呈高表达，在CINⅠ～Ⅱ组织有一定的表达，但在CINm及ICC中不表达，差异具有显著性（P〈0．05）；VEGF与高危HPV的表达存在正相关性，ER与高危HPV的表达无相关性，VEGF与ER表达亦无相关性。结论检测高危型HPV和VEGF是早期诊断CINHI及ICC是一个重要辅助方法，而ER表达缺失可能是宫颈癌发生的一个重要原因之一，联合检测HPV、VEGF及ER的表达，有助于探讨CIN及ICC的发病机制及提高诊断率，但CIN及宫颈癌的发病机制复杂，有待进一步研究。Objective To explore the expression of human papillomavirus （HPV）, vascular endothelial growth factor（VEGF） and estrogen receptor （ER） in the chronic cervicitis, cervical intraepithelial neoplasia（CIN） and invasive carcinoma of cervix （ICC） and the relation among HPV,VEGF and ER,in order to improve the the pre-operation diagnosis of CIN and ICC. Methods Immunohistochemical technique and in situ hybridization technique were employed to study the expression of HPV, VEGF and ER in 40 cases of chronic cervicitis,30 cases of CINⅠ～Ⅱ,25 cases of CINⅢ and 30 cases of ICC. HE stain was used in all cases. Results The high-risk HPV infection in the cervical tissue was closely related to the CIN and ICC, the expression of high risk HPV in the chronic cervicitis and CIN Ⅰ～Ⅱ was low and remarkably lower than that in CIN Ⅲ and ICC ,the difference was statistically significant（P 〈 0.05 ）. The expression of VEGF in the chronic cervicitis and CIN Ⅰ～Ⅱ was low and remarkably lower than that in CINⅢand ICC ,the difference was statistically significant（P 〈 0.05 ）. The expression of ER in the chronic cervicitis and CIN Ⅰ～Ⅱ was higher than that in CIN Ⅲ and ICC and the difference was statistically significant （P 〈 0.05 ）. The expression of high risk HPV was positively correlated with the expression of VEGF ; and was not correlated with the expression of ER. The expression of VEGF was also not correlated with the expression of ER. Conclusions It is an important auxiliary way to diagnose the CIN and ICC by detecting the high risk HPV and VEGF and no expression of ER may be an important cause of cervical cancinogenesis by detecting the expression of HPV, VEGF and ER is useful for studying the mechanism and improving the diagnosis of CIN and ICC, but more researches are needed for elucidating the pathogenesis of CIN and ICC.

关 键 词：HPV VEGF ER 宫颈上皮内瘤变 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤] R730.45[医药卫生—临床医学]