ICP患者外周血单个核细胞Th1及Th2细胞变化  

Study on changes of Th1 and Th2 cells in peripheral blood mononuclear cell from patients with intraheptic cholestasis of pregnancy

作　　者：肖敏[1] 凌斌[1] 姚风球 周颖[2] 赵卫东[1] 陈铮铮[1] 程志祥[2] 沈国栋[2] 高宗侠[1] 

机构地区：[1]安徽医科大学附属省立医院妇产科,合肥230001 [2]安徽医科大学附属省立医院分子医学重点实验室,合肥230001

出　　处：《安徽医科大学学报》2007年第5期556-559,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科研基金资助项目(编号:34071805)

摘　　要：目的研究妊娠期肝内胆汁淤积症(ICP)患者Th1和Th2型细胞免疫反应的变化,从免疫学角度探讨ICP发病机制。方法采用流式细胞术,分别检测了20例重度ICP患者与20例正常晚期妊娠妇女的外周血单个核细胞和T细胞中Th1和Th2细胞所占的比例。结果重度ICP组外周血单个核细胞中IFN-γ+细胞比例为17.82%±3.43%,明显高于正常晚期妊娠组12.66%±2.34%(P<0.01),而IL-4所占的比例为1.17%±0.26%,低于正常晚期妊娠组1.37%±0.30%,差异有显著性(P<0.05);重度ICP组PB-MC中细胞因子Th1/Th2比率为16.00±4.66,显著高于正常晚期妊娠组9.89±2.82(P<0.01);重度ICP组外周血中T细胞分泌的Th1细胞比例为14.37%±1.38%,明显高于正常晚期妊娠组10.66%±1.18%(P<0.01),而Th2细胞比例1.05%±0.19%,低于正常晚期妊娠组1.24%±0.29%,差异有显著性(P<0.01);重度ICP组T细胞Th1/Th2比率为13.99±2.46,显著高于正常晚期妊娠组8.94±1.84(P<0.01)。结论重度ICP患者与正常晚期妊娠妇女比较,其体内表现为Th1型免疫反应增强,而Th2型免疫反应减弱。体内Th1/Th2细胞平衡紊乱可能与妊娠期肝内胆汁淤积症发生相关。Objective Changes of Thl and Th2 cell immune in the peripheral blood from patients with ICP were investigated in this paper, in order to further explore the immunological etiology of intrahepatic cholestasis of preg nancy （ICP）. Methods With peripheral blood mononuclear cells （PBMC） from 20 patients with severe ICP and 20 third pregnant women separated, we examined cell percentage of Thl cells and Th2 cells in PBMC and T cells by flow cytometry. Results The percentage of IFN-γ PBMC from severe ICP patients was 17.82% ± 3.43%, significantly higher than that of normal third women which was 12. 66% ±2. 34% （P 〈0. 01 ）. However, the percentage of IL-4 was 1.17% ± 0. 26%, much lower than that of normal third women 1.37% ± 0. 30% （ P 〈 0. 05）. The ratio of Thl/Th2 cells PBMC was elevated（P 〈0. 01 ）. Similarly, 14. 37% ± 1.38% of T cells from severe ICP patients secreted IFN-γ, while so did only 10. 66% ± 1.18% of T cells in third pregnant women. Between them, there was significant difference （ P 〈 0. 01 ）. 1.05% ± 0. 19% of IL-4 ^＋ T cells was lower, compared to 1.24% ± 0. 29 % of third pregnant women （P 〈 0. 05 ）, and the ratio of Th1/Th2 cells was elevated significantly （P 〈0. 01 ）. Conclusion Compared with pregnant women of the normal third trimester,Thl type immune reactions in severe ICP patients are elevated, which lead to immune damage and weaken Th2 type immunoreactions which is immunelprotection . Imbalance of Thl/Th2 might correlate with intrahepatic cholestasis of pregnancy.

关 键 词：流式细胞术 胆汁淤积 肝内/并发症 妊娠并发症 TH1细胞 TH2细胞 

分 类 号：R575.06[医药卫生—消化系统] R657.43[医药卫生—内科学]