肝癌组织中ras、c-myc、c-erbB-2和p53的蛋白表达  

Expression of ras,c myc,c erbB 2 and p53 proteins hepatocellular carcinoma tissues

作　　者：汪荣泉[1] 周子成[1] 杨建民[1] 房殿春[1] 晋华源[1] 

机构地区：[1]第三军医大学西南医院消化科

出　　处：《临床与实验病理学杂志》1997年第1期4-6,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：探讨肝癌的发病机理。方法：应用免疫组化ＳＰ法对４６例肝细胞癌组织及其３８例癌旁组织中癌基因ｒａｓ、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２和抑癌基因ｐ５３的蛋白表达作了定位研究。结果：在癌组织中的检出率分别为５８．７％、６７．４％、４７．８％和２０．４％，癌旁组织中的检出率分别为３４．２％、４７．４％、４２．１％和７．９％。ｒａｓｐ２１蛋白位于肝细胞和肝癌细胞的胞浆内；ｃ－ｅｒｂＢ－２蛋白位于肝细胞和肝癌细胞的胞浆和胞膜；ｃ－ｍｙｃ蛋白位于肝细胞和肝癌细胞的胞核内；ｐ５３蛋白位于肝细胞的胞浆和癌细胞的胞核内。ｐ５３蛋白表达与ｒａｓ、ｃ－ｅｒｂＢ－２蛋白表达有显著的协同性（Ｐ＜０．０５），与ｃ－ｍｙｃ蛋白表达无协同性（Ｐ＞０．０５）。结论：肝细胞癌的发生可能与癌基因ｒａｓ、ｃ－ｍｙｃ、ｃ－ｅｒｂＢ－２的激活和抑癌基因ｐ５３的失活密切相关，部分基因的改变存在协同性。Purpose To study the pathogenesis of hepatocellular carcinoma. Methods Expression of ras,c myc,c erbB 2 and p53 proteins in the cancerous tissues of 46 patients with hepatocellular carcinoma(HCC) and its 38 peri cancerous tissues was studied with the streptavidin peroxidase conjugataed method (SP method). Results The positivity rates of ras, c myc,c erbB 2 and p53 proteins were 58 7%,67 4%,47 8% and 20 4% in HCC group and 34 2%,47 4%,42 1% and 7 9% in peri cancerous group respectively. The ras p21 was located in the cytoplasms of hepatocytes and HCC cells, c erbB 2 protein in the cytoplasms and on the cell membranes of hepatocytes and HCC cells, c myc protein in the nuclei of hepatocytes and HCC cells, p53 protein in the cytoplasms of hepatocytes and in the nuclei of HCC cells. The expression of p53 protein had significant synergy with the expression of ras and c erbB 2 proteins (P<0 05) , but no synergy with the expression of c myc protein. Conclusions The results indicated that ras, c myc, c erbB 2 and p53 are related with HCC occuring. They may play a role in HCC occuring.

关 键 词：肝细胞癌 癌基因 抑癌基因 肝肿瘤 

分 类 号：R735.702[医药卫生—肿瘤]