Bcl-2反义寡核苷酸对放射治疗诱导肺癌细胞凋亡的促进作用  

Bcl-2 Antisense Oligodeoxynucleotides Increase Apoptosis of Lung Cancer Cells Induced by Radiation

作　　者：胡炜[1] 朱成楚[1] 朱敏[1] 陈葆国[1] 单国平[1] 余长辉[1] 

机构地区：[1]浙江省台州医院放疗科,317000

出　　处：《医学研究杂志》2007年第11期59-62,共4页Journal of Medical Research

摘　　要：目的通过体外试验,研究Bcl-2反义寡核苷酸对放射治疗诱导肺癌细胞凋亡的促进作用。方法NCI-H446肺癌细胞株分5组,分别为空白对照、单纯照射组、脂质体加照射组、无义序列加照射组、反义寡核苷酸加照射组。培养至6h、12h、24h、48h和72h,分别收集各组细胞,瑞吉染色做细胞形态学分析,应用RT-PCR半定量测定p53、Bcl-2和PTEN基因的mRNA表达,流式细胞仪检测各组细胞DNA倍体,分析细胞凋亡指数。结果Bcl-2反义寡核苷酸联合照射后p53和PTEN表达明显增加,Bcl-2mRNA表达则明显降低。流式细胞仪检测照射后72h空白对照组、照射组、脂质体加照射组、无义序列加照射组、反义寡核苷酸加照射组凋亡率分别为0.14±0.09,13.17±2.47,11.84±1.76,13.72±1.4,21.26±2.97,反义寡核苷酸加照射组与其余4组比较差别有显著性意义(P<0.01)。结论Bcl-2反义寡核苷酸对放射治疗诱导肺癌细胞凋亡有促进作用;细胞凋亡受p53和Bcl-2基因调控,p53基因的表达促进了细胞的凋亡,而Bcl-2基因的高表达抑制细胞的凋亡,PETN的表达与凋亡也有相关性。Objective To study the effects of Bcl - 2 antisense oligodeoxynucleotides （ASODN） on the apoptosis of lung cancer cells induced by radiation in vitro. Methods NCI - H446 lung cancer cell strains were divided into 5 groups：control simple radiation, lipofectin plus radiation, nonsense sqnence radiation and ASOI）N plus radiation. The cells cultured in five groups were collected at 6h, 12h, 24h ,48h and 72h ,with Wright- Giemsa stain, morphology analysis for which was done;the mRNA expression for p53 ,bcl -2 and PTEN gene was examined by RT - PCR half quantivity and DNA - ploid of the cells in five groups was detected by flow cyfometric method. Results Cell proliferation is obviously restrained and conformation is changed too with the shape crimpled and adherence function decreased obviously after irradiated for 10 Gy dose by the linac; p53 and PTEN expression clearly increased for the combination of Bcl -2 ASODN and bcl - 2 mRNA expression clearly decreased. The apoptosis rate after 72 hours among control, pure radiation, lipofectin ＋ radiation, nonsense ＋ radiation and ASODN ＋ radiation grouop is 0.14 ± 0.09,13.17 ± 2.47,11.84 ± 1.76,13.72 ± 1.4,21.26 ± 2.97 respectively, the difference between ASODN combined with radiation grouop and other 4 groups are significant（P 〈 0.01 ）. Conclusions Bcl - 2 ASODN can significantly increase apoptosis of lung cancer cells induced by radiation . The cell apoptosis is regulated by p53 , Bcl - 2 and PTEN gene after the NCI - H446 cell is irradiated .

关 键 词：BCL-2 反义寡核苷酸 放射治疗 肺癌 凋亡 

分 类 号：R817.42[医药卫生—影像医学与核医学]