PPARγ在肝细胞癌组织中的表达及临床意义  

The expression and clinical significance of PPARγ in hepatocellular carcinoma

作　　者：吴旭东[1] 陈卫昌[1] 张素青[2] 

机构地区：[1]苏州大学附属第一医院消化病科,215006 [2]南通市肿瘤医院肝胆外科

出　　处：《江苏医药》2007年第11期1106-1109,F0002,共5页Jiangsu Medical Journal

摘　　要：目的探讨过氧化物酶体增殖物活化受体γ(PPARγ)在肝细胞癌(HCC)中表达的临床意义。方法半定量RT-PCR法、免疫组织化学法检测34例患者HCC组织及相对应的癌旁组织的PPARγ表达,结合临床特征行统计学分析。结果79.4%(27/34)HCC组织PPARγmRNA表达高于癌旁组织。PPARγ蛋白在HCC组织中均呈阳性表达,表达在胞浆和(或)胞核中;而癌旁组织为阴性或弱阳性表达,为胞浆染色。阳性表达分级之间存在显著差异,χ2=39.62,P<0.05。PPARγmRNA表达水平与蛋白表达分级与HCC大小、包膜完整、高转移复发倾向和HBV感染及肝硬化背景密切相关(P<0.05);但与患者年龄、性别、Okuda分期、pTNM分期、组织分化程度、AFP阳性和随访转移结果无关。PPARγmRNA及蛋白高表达均与患者的总生存期负相关。结论PPARγ在HCC中过表达,与HCC的发展可能有关,可能作为判断HCC预后的一种预测性指标。Objective To explore the expression and clinical significance of peroxisome proliferators-activated receptor gamma （PPARγ） in hepatocellular carcinoma （HCC）. Methods PPARγ mRNA was detected with half quantitation reverse transcriptase PCR （RT-PCR） and immunohistochemical method in 34 cases, including HCC tissues and corresponding peri-tumor tissues, and the clinical significance of PPARγ was analyzed. Results PPARγ mRNA in HCC tissues was higher than that in peri-tumor tissues in 27 cases（79.4%）. All of the HCC tumor cells stained positively for PPARγ protein, which were expressed in cytoplasm and/or nucleus of hepatocytes. Hepatocytes of peri-tumor were stained negative or weakly positive in cytoplasm. The difference of the grade of positive staining between two groups was significant（P〈0. 05）. The clinical characteristics of HCC, such as size of tumor, integrity of tumor amicula, high-trend metabasis, the background of HBV infection and cirrhosis, were closely correlated with the expression of PPARγ mRNA and grade of positive staining, but the patients＇ ages, gender, Okuda and pTNM stages, serum AFP levels, degrees of histological differentiation and metabasis rate by follow up were not. There was a negative correlation between the expression of PPARγ and the total life span in HCC patients. Conclsion PPARγ overexpression in HCC is associated with the carcinogenesis and development of HCC, and may be taken as an index for predicting the prognosis of HCC.

关 键 词：过氧化物酶体增殖物活化受体Γ 肝细胞癌 生存分析 

分 类 号：R735[医药卫生—肿瘤]