DPC4/Smad4在胰腺癌组织中的表达  

Expression of DPC4/Smad4 in the specimens of human pancreatic carcinoma

作　　者：沈伟[1] 李德春[2] 白霞 朱兴国[2] 陶国清[1] 

机构地区：[1]无锡市第一人民医院普通外科,214002 [2]苏州大学附属第一医院普通外科 [3]江苏血液研究所血栓室

出　　处：《江苏医药》2007年第11期1129-1131,F0003,共4页Jiangsu Medical Journal

摘　　要：目的检测胰腺癌组织和正常胰腺组织中DPC4/Smad4基因的表达差异,探讨其在胰腺癌发病机制中的作用。方法分别运用RT-PCR和免疫组化技术检测40例胰腺癌和36正常胰腺组织中的DPC4mRNA及蛋白的表达。结果胰腺癌组织中DPC4阳性16例(40%);正常胰腺组织的DPC4的阳性表达率100%(36/36)。免疫组化显示癌组织中的弥漫阳性数为4例,局灶阳性数为10例,阳性表达率35%(14/40);正常胰腺组织的弥漫阳性数为16例,局灶阳性数为20例,阳性表达率100%(36/36)。两者比较有显著性差异(P<0.05)。结论DPC4基因在胰腺癌组织中有明显的表达异常,可能与胰腺癌的发病有一定的关系,可考虑作为胰腺癌患者一个临床诊断或预后的指标。ObJective To detect the expression of DWA gene in pancreatic adenocarcinoma and normal pancreatic specimens and discuss the role of DWA gene in the development of human pancreatic carcinoma. Methods The DPC4/Smad4 expression was determined in 40 pancreatic adenocarcinoma and 36 normal pancreatic specimins by RT-PCR assay and immunohistochemistry respectively. Results RT-PCR assay showed that positive rate of DPCA mRNA accounted for 100% （36/36） in all normal specimens, whereas 40% （16/40） in adenocarcinoma specimens. The regional and intense positive cases expressed DPCA protein in cancer tissues were 10 and 4, respectively, with a total positive rate of 35% （14/40）. All specimens of normal tissues expressed DPCA. Conclusion DPC4 gene expression between the normal and malignant pancreatic specimens is different, suggesting that DPCA gene may be involved in the development of pancreatic carcinoma and may be taken as a diagnostic and prognostic indicator for the patients with pancreatic cancer.

关 键 词：胰腺癌 DPC4/Smad4基因 

分 类 号：R735[医药卫生—肿瘤]