鼻咽癌细胞系IκBα mRNA表达及其DNA序列分析  

IκBα mRNA expression and its DNA sequence in nasopharyngeal carcinoma cell lines

作　　者：王光平[1] 陈方平[1] 付敢[1] 

机构地区：[1]中南大学湘雅医院血液科,长沙410008

出　　处：《中南大学学报（医学版）》2007年第5期758-764,共7页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金项目(30070945)~~

摘　　要：目的:探讨核转录因子κB抑制蛋白α(inhibitorα of nuclear transcription factorκB,IκBα) mRNA在鼻咽癌细胞系CNE1,CNE2,HNE1和HNE2细胞内的表达并对其DNA序列进行分析。方法:分别从鼻咽癌细胞系CNE1,CNE2,HNE1和HNE2细胞以及HNE1鼻咽癌移植瘤组织中提取细胞总RNA并逆转录成cDNA。随后,用PCR扩增IκBαcDNA并将其产物用于IκBα mRNAa表达分析和DNA序列直接测定或克隆到质粒载体后再测其序列。结果:在鼻咽癌细胞系CNE1,CNE2,HNE1和HNE2细胞内均可检测到IκBα mRNA表达。DNA序列测定结果显示,在HNE1鼻咽癌细胞移植瘤以及CNE1和CNE2鼻咽癌细胞内IκBαcDNA存在DNA多态性以及A825G,A975G,G576A,A655G和C653A基因突变。结论:在鼻咽癌细胞内不仅存在IκBα mRNA的表达,而且其cDNA还存在DNA多态性和基因突变。Objective To examine the expression of the inhibitor ct of nuclear transcription factor KB （IκBα） mRNA expression and its sequence characteristics in human nasopharyngeal carci-noma cell （NPC） lines CNE1, CNE2, HNE1 and HNE2. Methods Reverse transcription was performed with the total RNAs isolated from the NPC cell lines CNE 1 , CNE2, HNE 1 and HNE2, as well as the transplanted tumor tissues with HNE1 cells. Then IKBct cDNA was amplified by PCR, and the products were used to examine IκBα mRNA expression and DNA sequencing, or the DNA sequencing after the products were cloned into plasmid vector. Results IκBα mRNA was expressed in all the 4 nasopharyngeal carcinoma cell lines. DNA sequencing showed that polymorphisms and 5 mutations （A825G, A975G, G576A, A655G and C653A） existed in IKB cDNA from the trans-planted tumor tissues with HNE1 cells, CNE1 and CNE2 cells. Conclusion The expression of IκBα mRNA not only exists, but DNA polymorphisms and some additional mutations in IκBα cDNA are also detected in the nasopharyngeal carcinoma cells.

关 键 词：鼻咽癌 表达 基因突变 IΚBΑ CDNA 

分 类 号：R739.63[医药卫生—肿瘤]