选择性COX-2抑制剂NS-398对宫颈癌细胞的作用及其作用机制  

Mechanism and effect of selective cyclooxygenase-2 inhibitor NS-398 on human cervical carcinoma cells

作　　者：李新国[1] 吴敏[2] 张瑜[1] 

机构地区：[1]中南大学湘雅医院妇产科 [2]湖南省妇幼保健院妇产科,长沙410008

出　　处：《中南大学学报（医学版）》2007年第5期877-882,共6页Journal of Central South University ：Medical Science

摘　　要：目的:研究选择性COX-2抑制剂NS-398对宫颈癌细胞的作用及其作用机制,探讨NS-398与卡铂在宫颈癌治疗中的协同作用。方法:用NS-398、卡铂及两者联合作用人宫颈癌Hela细胞,四甲基偶氮唑盐(MTT)比色法检测NS-398、卡铂及两者联合对宫颈癌细胞增殖的影响,流式细胞仪PI染色法分析NS-398、卡铂处理后宫颈癌细胞凋亡率和细胞周期的改变。结果:NS-398、卡铂对人宫颈癌hela细胞的增殖具有抑制作用。卡铂联合NS-398对人宫颈癌hela细胞的增殖抑制作用显著增加,其联合抑制率近似于NS-398和卡铂单药抑制率之和,也近似于2倍卡铂剂量时的增殖抑制率。流式细胞仪检测发现,与对照组比较,NS-398处理组G1期细胞明显增加,而S期细胞明显减少,两组比较差异有统计学意义(P<0.05);而卡铂处理组G1期细胞明显减少,S期细胞明显增加,两组比较有统计学意义(P<0.05)。NS-398(200μmol/L)作用Hela细胞48h后,流式细胞仪检测发现,NS-398处理组凋亡率为3.43%±0.02%,对照组凋亡率为1.48%±0.03%,两组比较差异无统计学意义(P>0.05)。而卡铂(80mg/L)处理组凋亡率为9.32%±0.02%,与对照组比较差异有统计学意义(P<0.05)。结论:选择性COX-2抑制剂NS-398对宫颈癌细胞的增殖具有抑制作用。NS-398对宫颈癌细胞的作用可能与凋亡无关。NS-398与卡铂在宫颈癌化疗中具有叠加作用。Objective To investigate the effect and mechanism of the selective COX-2 inhibi-tor NS-398 and carboplatin on the human cervical carcinoma cell line Hela. Methods The effect of NS-398, carboplatin, and both on the proliferation of Hela cells was assessed by methylthiazolete trazolium （MTT） method, and the apoptosis assay and cell cycle distribution were analyzed by flowcytometry. Results NS-398, and carboplatin inhibited the growth of Hela cells in a dose and time-dependent manner. When combining carboplatin with NS-398, the combined inhibition rate was increased, which nearly equaled the inhibition rate of the double concentrations of carboplatin. Flow cytometry demonstrated that the cell cycle was redistributed： the G1-phase cell fraction was increased while the S-phase cell fraction was significantly decreased after the cells were treated with NS-398 （P 〈 0.05）, However, the result was just the opposite after being treated with carboplatin. The apoptotic rate was 1.48%±0.03% and 3.43%±0. 02% for pre-treatment and post-treatment with NS-398 respectively （P 〉 0.05） while the apoptotic rate was 9.32 %±0.02 % after the treatment with carboplatin （P 〈 0.05）. Conclusion NS-398 can inhibit the growth of Hela cells. The effect of NS-398 on Hela cells may not be related to the apoptosis. NS-398 and carboplatin can bring about synergistic effect in chemotherapy on Hela cells.

关 键 词：COX-2抑制剂 NS-398 宫颈癌 增殖 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]