几种简单易行的EB病毒转化外周血淋巴细胞建系方法的比较  

Comparison of Several Methods of Transformin Human B Lyphocyte by EB virus

作　　者：赵苏瑛[1] 李岷[1] 李克涓[1] 

机构地区：[1]江苏省中医院检验科,江苏南京210029

出　　处：《海南医学》2007年第12期154-156,共3页Hainan Medical Journal

摘　　要：目的比较几种EB病毒建系方法的可行性。方法利用EB病毒(Epstein-Barrvirus,EBV)转化淋巴细胞建系的四种方法:即微量全血法,冻存全血法,冻存白细胞法和环孢霉素A(CyA)法,对全血淋巴细胞进行转化建系。结果四种方法均可成功建系,按建系效率从高到低依次为:环孢霉素A法、冻存白细胞法、冻存全血法和微量全血法。结论在血量充足的情况下,建议选择环孢霉素A法进行建系。Objective Compare the feasibility of several methods in EB virus transforming B ceils. Methods Use four methods of transforming human B lymphocyte into immortal lymphoblastoid cell lines： small amout of whole blood method, frozen whole blood method, frozen leukocyte method and cyclosporine A method. Results All these methods can be used to establish the ceil lines successfully, arrange an order according to the efficiency, from high to low, is cyclosporine A method; frozen leukocyte method; frozen whole blood method; small amout of whole blood method. Discuss If the sample is enough, we recommend cyclosporine A method.

关 键 词：EB病毒转化 外周血淋巴细胞 建系方法 微量全血法 环孢霉素A 转化淋巴细胞 四种方法 细胞法 

分 类 号：R735.340.3[医药卫生—肿瘤]