牛磺酸对肾性高血压大鼠主动脉肥厚与组织局部肾素-血管紧张肽系统的影响  

Effect of Taurine on the Hypertrophy and Local Renin-Angiotensin System of the Aorta in the Rat with a Model of Renovascular Hypertension

作　　者：余良主[1] 化长林[1] 魏劲波[2] 王帮华[1] 

机构地区：[1]咸宁学院医学院生理学教研室,湖北437100 [2]武汉大学医学院生理学教研室,430071

出　　处：《医药导报》2007年第12期1406-1408,共3页Herald of Medicine

摘　　要：目的探讨牛磺酸对肾血管性高血压大鼠主动脉肥厚及组织局部肾素-血管紧张肽系统(RAS)的影响。方法W istar大鼠60只,随机分为3组,每组20只:假手术对照组、高血压对照组、牛磺酸治疗组。采用两肾一夹型肾血管性高血压大鼠模型。牛磺酸治疗组于术后第5周开始,灌胃给予牛磺酸50 mg.kg-1.d-1。采用标准尾套法间接检测清醒大鼠血压。给药8周后处死大鼠,分离其胸主动脉以用于检测胸主动脉的血管紧张肽Ⅱ含量(放射免疫法检测)、血管紧张肽转换酶活性(分光光度计法检测)和胸主动脉中膜厚度及中膜截面积。结果与假手术对照组大鼠相比,高血压对照组大鼠血压明显升高,胸主动脉的血管紧张肽Ⅱ含量增加,血管紧张肽转换酶活性增高,主动脉中膜厚度及中膜截面积明显增大。应用牛磺酸治疗后则明显抑制大鼠血压的升高,降低胸主动脉的血管紧张肽Ⅱ含量和血管紧张肽转换酶活性,并缩小主动脉中膜厚度及中膜截面积。结论长疗程牛磺酸治疗可抑制肾性高血压大鼠主动脉肥厚的发生,其机制可能与抑制主动脉组织局部血管紧张肽Ⅱ生成有关。Objective To study the effect of taurine on the hypertrophy and local renin-angiotensin system （RAS） of the aorta in rats with renovascular hypertesion. Methods Sixty male Wistar rats were randomly divided into 3 equal groups： （1）the sham-operation group, （2）hypertension control group and （3） taurine treatment group. A model of renovascular hypertersion was set up in each of the rats of group（2） and group （3） with the two-kidneys-one-clip method in which the left renal artery was partially constricted with a silver clamp. 5 weeks after the operation, rats of group （3） were given each 50 mg · kg^-1 of taurine adminislered by gastrogavage q.d. for 8 consecutrve weeks. Rats of group（1） and group （2）were given each an equivalent amount of distilled water with the same method and for the same duration of time. Blood pressure was measured in conscious rats with the standard tail-cuff method. At the termination of the 8 week medication, the rats were sacrificed by decapitation and specimens of the thoracic aorta were prepared for the determination of the content of angiotensin Ⅱ with radioimmunoassay and the activity of angiotensin-converting enzyme with spectrophotometry as well as for the measurement of the thickness and cross-sectional area of the aortic media in a color image analysis system. Results Compared with rats of the sham-operation group, animals of the hypertention control group exhibited a striking elevation of the blood pressure, and a dramatic increase in the content of angiotensin Ⅱ and the activity of angiotensin-converting enzyme in the thoracic aorta while the media thickness and media crosssectional area of the aortic media were also significantly increased. Taurine was shown to significantly inhibit the elevation of the blood pressure, decrease the content of angiotensin Ⅱ and activity of angiotensin-converting enzyme in the thoracic aorta and decrease the thickness and cross-sectional area of aortic media in rats of groups （3） as compared with thos

关 键 词：牛磺酸 高血压 主动脉 血管肥厚 血管紧张肽Ⅱ 血管紧张肽转换酶 

分 类 号：R972.4[医药卫生—药品] R544.1[医药卫生—药学]