MD-2基因启动子区-1064、-1625 SNP对启动子活性的影响  

Effect of -1064,-1625 SNP on MD-2 promoter activity

作　　者：单佑安[1] 刘庆[1] 董蕻[1] 顾玮[1] 蒋建新[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第四研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《第三军医大学学报》2007年第3期185-187,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目("973"项目)(2005CB522602);国家杰出青年科学基金(30325040)~~

摘　　要：目的探讨MD-2基因启动子区-1064、-1625SNP位点碱基变异对启动子活性的影响。方法通过PCR和定点突变技术,构建了含MD-2基因基础启动子及-1625G和-1064G突变启动子,以双荧光素酶报告系统观察SNP对MD-2基因启动子活性的影响。结果化学发光结果显示,在U937细胞中,-1625G突变启动子活性比MD-2基础启动子活性高11.4%,差异显著。而-1064G突变启动子与MD-2基础启动子活性无明显差异。结论-1625C→G碱基变异可增强MD-2基因启动子活性,但-1064SNP对靶基因启动子活性无明显影响。Objective To study the effect of - 1064, - 1625 single nucleotide polymorphisms （SNPs） on MD-2 promoter activity. Methods PCR and site direct mutation technology were used to construct MD-2 basic promoter and -1064 G, -1625 G mutate promoter. Dual-Luciferase Reporter assay system was used to detect the activity of constructed promoter. Results In U937 cells, the activity of - 1625 G mutate promoter was higher than - 1625C promoter, and there was no significant difference between - 1064A promoter and - 1064 G promoter activity. Conclusion It is implied that - 1625 C→G base variation can enhance the activity of MD-2 promoter, while - 1064 SNP has no effect on MD-2 promoter activity.

关 键 词：MD-2 启动子 单核苷酸多态性 

分 类 号：R394.33[医药卫生—医学遗传学] R394.3[医药卫生—基础医学]