戊地昔布的抑瘤作用与COX-2的表达及活性的关系  

Relationship between antitumor effect of valdecoxib and COX-2 expression

作　　者：李军霞[1] 杲海霞[1] 苏素文[1] 王永利[1] 

机构地区：[1]河北医科大学基础医学部药理学教研室,石家庄050017

出　　处：《第三军医大学学报》2007年第3期243-246,共4页Journal of Third Military Medical University

摘　　要：目的探讨戊地昔布对肿瘤的抑制作用及其与COX-2是否有关。方法用Westernblotting和免疫细胞化学检测细胞COX-2的表达。用MTT法测定药物对细胞增殖的影响。用ELISA测定PGE2(前列腺素E2)含量。结果①BGC-823、HGC-27和SK-OV-3细胞均无COX-2表达,clone26COX-2表达阳性。②戊地昔布可明显抑制4种肿瘤细胞的生长,对BGC-823、HGC-27、SK-OV-3和clone26生长抑制的IC50分别为110.7、99.2、113.3、117.6μmol/L。③3种药物对BGC-823和clone26细胞生长抑制程度由高到低的顺序,为戊地昔布、SC-560(选择性COX-1抑制剂)和吲哚美辛(非选择性COX-2抑制剂)。④PGE2本身对BGC-823和clone26细胞的生长没有影响,也不拮抗戊地昔布、SC-560和吲哚美辛对细胞的生长抑制作用。⑤戊地昔布和吲哚美辛抑制细胞生长作用和抑制COX-2的作用不平行,在不影响细胞生长的浓度时就明显降低clone26上清的PGE2含量。结论戊地昔布抑制肿瘤细胞生长的作用与COX-2无关。Objective To evaluate the inhibitory effect of valdecoxib on the growth of the cancer cell lines and involvement of COX-2 in this inhibition, Methods Western blotting and immunocytochemistry were used to detect the expression of COX-2, MTF assay was used to determine inhibitory effect of the drugs on the cell growth. The content of PGE2 in cell medium was determined with PGE2 ELISA kit, Results①Clone 26 cells expressed high levels of COX-2, whereas BGC-823, HGC-27 and SK-OV-3 cell had no COX-2 expression. ②Valdecoxib inhibited the growth of BGC-823, HGC-27, SK-OV-3 and clone 26 cells, with a IC50 of 110, 7, 99, 2, 113, 3, 117,6 μmol/L, respectively, ③The inhibitory effect of these drugs on BGC-823 and clone 26 cell was in the descending order of valdecoxib, SC-560 and indomethacin. ④PGE2 did not antagonize the effect of valdecoxib, SC-560 and indomethacin on BGC-823 and clone 26 ceils, ⑤The and indomethacin on the growth of clone 26 cells was not compatible with that on hibitory effect of valdecoxib on cell growth is not related to its effect on COX-2. inhibitory effect of valdecoxib PGE2. Conclusion The in-hibitory effect of valdecoxib on cell growth is not related to its effect on COX-2.

关 键 词：戊地昔布 肿瘤 COX-2 SC-560 吲哚美辛 

分 类 号：R73-36[医药卫生—肿瘤] R965[医药卫生—临床医学]