不同剂量环磷酰胺对裸鼠A549细胞移植瘤凋亡的影响  

Effect of low-dose cyclophosphamide on cell apoptosis of implanted human lung carcinoma in nude mice

作　　者：盛伟利[1] 梅同华[1] 

机构地区：[1]重庆医科大学附属第二医院呼吸内科,重庆400010

出　　处：《第三军医大学学报》2007年第4期311-313,共3页Journal of Third Military Medical University

基　　金：重庆市卫生局资助项目(200453)~~

摘　　要：目的探讨小剂量环磷酰胺对裸鼠肺腺癌移植瘤凋亡的影响。方法建立裸鼠人肺腺癌移植瘤模型,不同剂量环磷酰胺干预,测定抑瘤效果和副作用,免疫组化检测NF-κBP65表达,电镜、TUNEL检测肿瘤细胞凋亡情况。结果小剂量组经环磷酰胺处理后肿瘤体积与大剂量组和对照组相比明显减小;电镜下出现典型凋亡小体,凋亡指数明显增高(P<0.05)而P65蛋白表达比对照组显著减少(P<0.05)。结论小剂量环磷酰胺能抑制肺癌移植瘤增长,诱导肿瘤细胞凋亡,可能与下调NF-κB信号传导途径有关。Objective To explore the effect of low-dose cyclophosphamide （CTX） on apoptosis of implanted human lung carcinoma in nude mice. Methods BALB/c nude mice bearing lung carcinoma were randomized into several groups, each receiving different dose of CTX respectively. We investigated antitumor effect and toxicity of CTX. At the end of experiment, tumors were resected for immunohistochemical staining. Nuclear factor κB （NF-κB P65 ）was detected and apoptosis was measured by TUNEL. Morphological changes of tumor cells were observed. Results The volume of tumors was much smaller in mice receiving continuous low-dose CTX than that receiving maximally tolerated dose of CTX and normal saline （ control group）. The apoptotic index was increased and NF-KB P65 decreased in the mice receiving low-dose of CTX. Conclusion The continuous low-dose of CTX inhibits the growth of implanted tumor and increases tumor cell apoptosis, which may be due to the down-regulation of nuclear factor KB.

关 键 词：肺肿瘤 凋亡 环磷酰胺 

分 类 号：R73-361[医药卫生—肿瘤] R734.2[医药卫生—临床医学]