不同培养条件对银杏愈伤组织生长及黄酮含量的影响  

Effects of Different Culture Conditions on Flavone Growth and Content of Ginkgo biloba Callus

作　　者：朱红威[1] 邵菊芳[1] 李庆[1] 王丹[1] 

机构地区：[1]中国矿业大学化工学院,江苏徐州221008

出　　处：《食品科学》2007年第11期102-105,共4页Food Science

基　　金：中国矿业大学科技基金资助项目(OH060101;OH061009)

摘　　要：以胚为外植体,通过固体培养,研究了培养基,激素和光照对银杏愈伤组织诱导的影响,以及继代培养中激1素和活性炭对愈伤组织生长和产黄酮的影响,结果表明:愈伤组织的诱导的最佳条件为DCR培养基+1.0mg/L2,4-D+0.5mg/LBA+完全暗条件培养。继代培养过程中既能促进愈伤组织生长又使黄酮含量最高的处理为0.3mg/LBA+2.0mg/L2,4-D,活性炭抑制愈伤组织生长但能促进黄酮的积累。The effects of solid media, hormone and light for inducement from Ginkgo biloba embryo to callus growth were studied. The effects of hormone and carbon for callus growth and flavone output were studied as well. The results showed that： （1） The explant cultured on DCR medium with 1.0 mg/L 2,4-D ＋0.5 mg/L BA in dark is of the optmum conditions to induce callus growth. （2） 0.3 mg/L BA＋2.0 mg/L 2,4-D hormone not only accelerate callus growth but also heighten flavone contents in seccedent culture.（3） Carbon inhibitates callus growth, but enhances flavone accumulation.

关 键 词：培养条件 银杏 愈伤组织 黄酮含量 

分 类 号：S792.95[农业科学—林木遗传育种]