低分子量壳寡糖改善II型糖尿病大鼠症状及其作用机制研究  

Symptom and Mechanism of Low Molecular Chitooligosaccharides in II Type Diabetic Rats

作　　者：王鑫[1] 林强[1] 田平芳[2] 桑帅[1] 高伟[1] 

机构地区：[1]北京联合大学生物化学工程学院,北京100023 [2]北京化工大学生命科学与技术学院,北京100029

出　　处：《食品科学》2007年第11期529-532,共4页Food Science

基　　金：北京市教育委员会科技成果转化项目

摘　　要：目的:探讨不同分子量壳寡糖对糖尿病大鼠的降糖机制。方法:Wistar大鼠采用高脂高糖饮食同时腹腔注射链脲佐菌素复制II型糖尿病大鼠模型。将动物随机分为正常对照组、模型组、阳性对照和药物治疗组。四周后处死大鼠取血清测定血糖、果糖胺;取新鲜肝脏测定肝糖原、SOD、MDA。结果:与正常组比较,模型组血糖、果糖胺水平明显升高(p<0.01),肝糖元含量明显降低(p<0.05)。与模型组比较,治疗组各项指标有明显改善(p<0.05),且这种作用具有剂量和分子量依赖性。结论:本模型大鼠血糖升高,并引起自由基代谢紊乱,分子量1700的壳寡糖、大鼠日用剂量为140mg/kg适合高脂糖尿病患者,可降低血糖、提高抗氧化能力起到保健作用。Objective： To investigate the influence of low molecular chitooligosaccharides（COS） in diabetic rats. Methods：Diabetic model was induced by injecting streptozotocin （STZ） and the rats were treated with high fat and sugar diet for four weeks. Rats were randomly divided into four group： normal group, model group, positive control group and different COS treatment group. Blood sample was taken out from rats at the end of the experiments after 24 hours＇ fasting for determination of the blood glucose, fructosamine. Fresh liver was taken out for determination of the glycogen, SOD, MDA. Results： Comparing with the normal group, the blood glucose, fructosamine were obviously different in model group （p 〈 0.01）. The above mentioned index was improved （p 〈 0.05） at the end of the experiments in COS group. And the effect was determined by dosage and molecular. Conclusions： The best molecular of COS was 1700, and the dosage was 140 mg/kg · d in this diabetic model.

关 键 词：壳寡糖 糖尿病 肝糖元 超氧化物歧化酶 丙二醛 

分 类 号：R285.5[医药卫生—中药学]