新疆维吾尔族妇女子宫颈鳞状细胞癌中人乳头瘤病毒16感染和p33^(ING1b)及端粒酶逆转录酶催化亚单位表达的相关性  

Relationship between Human Papillomavirus 16 Infection and the Expression of p33^(ING1b),Human Telomerase Reverse Transcriptase in Cervical Squamous Cell Carcinoma of Uygur Female in Xinjiang Uygur Autonomous Region

作　　者：玛依努尔.尼亚孜 刘芳[1] 朱开春[2] 戴建霞[2] 阿也提.司马义 王琳[1] 昆多孜.乌赛那洪 

机构地区：[1]新疆维吾尔自治区人民医院妇科,乌鲁木齐830001 [2]新疆维吾尔自治区人民医院中心实验室,乌鲁木齐830001

出　　处：《中国医学科学院学报》2007年第5期592-596,共5页Acta Academiae Medicinae Sinicae

基　　金：新疆维吾尔自治区自然科学基金(200521107)~~

摘　　要：目的探讨新疆维吾尔族妇女子宫颈鳞状细胞癌组织中人乳头瘤病毒(HPV)16感染和p33ING1b及端粒酶逆转录酶催化亚单位(hTERT)表达的相关性。方法采用免疫组织化学方法检测了50例子宫颈鳞状细胞癌(SCC)和34例子宫颈上皮内瘤变(CIN)p33ING1b和hTERT表达情况,PCR法检测HPV16感染情况,并与12例正常子宫颈进行对照。结果对照组、CIN1组、CIN2～3组和SCC组中HPV16感染率分别为0、22.2%、44.0%和74.0%,各组间差异有显著性(x2=26.537,P=0.000);p33ING1b阳性率分别为91.7%、77.7%、68.0%和36.0%,各组间差异有显著性(x2=38.943,P=0.000);hTERT阳性率分别为50.0%、66.6%、88.0%和94.0%,各组间差异也有显著性(x2=48.199,P=0.000)。HPV16感染与p33ING1b表达呈负相关(r=-0.294,P=0.004),与hTERT表达呈正相关(r=0.286,P=0.005);p33ING1b表达与hTERT表达呈负相关(r=-0.361,P=0.000)。结论HPV16感染可能与新疆维吾尔族妇女子宫颈SCC组织中p33ING1b表达下降及hTERT表达增加有关。Objective To explore the relationship between human papillomavirus （HPV） 16 infection and the expression of p33^ING1b, human telomerase reverse transcriptase （hTERT） in cervical squamous cell carcinoma of Uygur Female in Xinjiang Uygur Autonomous Region. Methods Polymerase chain reaction （PCR） and immunohistochemical methods were used to detect HPV16 infection and the expression of p33^ING1b and hTERT in the normal control group （ n = 12）, the patients with cervical intraepithelial the patients with cervical squamous cell carcinoma （SCC） （ n = 50）. female, the HPV16 infection rate was 0 in control group, 22.2% neoplasm （CIN） （ n In the cervical tiss in the CIN 1 group, 44.0% =34）, and ues of Uygur in CIN 2 ＆ CIN 3 group, and 74.0% in SCC group （P =0. 000）. The expression rate of p33^ING1b decreased was 91.7% in control group, 77.7% in CIN 1 group, 68.0% in CIN 2 ＆ CIN 3 group, and 36. 0% in SCC group （ P = 0.000）. The expression rate of hTERT was 50.0% in control group, 66.6% in CIN 1 group, 88.0% in CIN 2 ＆ CIN 3 group, and 94.0% in SCC group （ P = 0. 000 ）. In the cervical tissues of Uygur female, the HPV16 infection rate was negatively correlated with the expression of p33^ING1b （ r = - 0. 294, P = 0. 004 ） , and was positively correlated with the expression of hTERT （ r = 0. 286, P = 0. 005 ）. The expression of p33^ING1b was negatively correlated with the expression of hTERT （r = -0. 361, P = 0. 000 ）. Conclusion The infection of HPV 16 correlates with the decreased expression of p33^ING1b and increased expression of hTERT in the cervical squamous cell carcinoma of Uygur female in Xinjiang.

关 键 词：子宫颈鳞状细胞癌 P33^ING1B 人端粒酶逆转录酶催化亚单位 人乳头瘤病毒16 维吾尔族 

分 类 号：R737.33[医药卫生—肿瘤]