柑橘裂皮类病毒广东分离物YC全长cDNA序列克隆及分析  

Cloning and Analyzing a Full Length cDNA of Citrus exocortis Viroid YC Isolate from Guangdong

作　　者：丁芳[1] 易干军[2] 王国平[1] 曹庆[2] 洪霓[1] 钟云[2] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]广东省农业科学院果树研究所,广州510640

出　　处：《分子植物育种》2007年第6期871-874,共4页Molecular Plant Breeding

基　　金：国家863课题(2001AA241142);广东省科技攻关项目(2003B21601);湖北省科技攻关计划(No.2006AA203B05)

摘　　要：柑橘裂皮类病毒(Citrus exocortis viroid,CEVd)是危害柑橘的重要病原之一,认清该类病毒的分子特性是进一步研究其致病机制的前提。本文利用RT-PCR技术对广东分离物YC的全长cDNA序列进行了扩增、克隆,获得了全长372 bp的片段,对该片段进行测序及分析显示:CEVd广东分离物YC的分子结构域包括5个功能区即左手末端区(TL)、致病区(P)、中央保守区(C)、可变区(V)和右手末端区(TR)。通过与GenBank已登陆序列的比对分析发现:与来自西班牙的分离物CEVd-f-1(登陆号:EF494677.1)的同源性高达98%;与湖北分离物CEVd-HB(登陆号:AY456136)的同源性仅为92.7%;与强度株系CEVd-A(登陆号:M30868)的同源性为96.8%,与弱毒株系CEVd-de26(登陆号:K00965)的92.4%。说明该广东分离物YC分子特性更接近强毒株系。这是国内首次对柑橘裂皮类病毒cDNA的分子特性进行报道。Citrus exocortis viroid causes one of the most severe diseases on citrus production. The understanding of its genomic molecular character would be a basis for further research of pathogenicity. In this research, a 372 bp full length cDNA sequence from citrus exocortis viroid isolate YC from Guangdong province was obtained using RT-PCR method. Cloning and sequencing analysis showed that there are totally 5 functional regions in obtained sequence including left hand terminal domain, pathogenic domain, central domain, variable domain and right hand terminal domain. The blastn analysis indicated that the cDNA has highly 98% homologies with CEVd-f-1 （Accession number： EF494677.1） which originated from Spanish and 92.7% lowly homologies with CEVd-HB from Hubei province （Accession number： AY456136）, and also the homologies of the obtained cDNA compared to severe strain CEVd-A （Accession number： M30868） and mild strain CEVd-de26 （Accession number： K00965） wereabout 96.8% and 92.4%, respectively. It would suggested that YC isolate from Guandong provice should be more likely a severe strain. This might be the first report for molecular character ofCEVd cDNA in China.

关 键 词：裂皮类病毒 全长CDNA 克隆 分析 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]