慢性乙型肝炎和肝硬化及肝癌与HLA-DQA1基因多态性关系的研究  

Association of HLA-DQA1 allele and chronic hepatitis B,liver cirrhosis and hepatocellular carcinoma

作　　者：刘春涛[1] 程宝泉[1] 张宗利[2] 

机构地区：[1]山东大学齐鲁医院消化内科,山东济南250012 [2]山东大学齐鲁医院普外科,山东济南250012

出　　处：《中国现代普通外科进展》2007年第5期424-426,共3页Chinese Journal of Current Advances in General Surgery

基　　金：山东省优秀中青年科学家科研奖励基金资助(03BS035)

摘　　要：目的:研究HLA-DQA1等位基因多态性与慢性乙型肝炎(HBV)病毒感染、肝硬化及肝癌的关系。方法:采用聚合酶链序列特异性引物(PCR-SSP)技术分别对168例慢性HBV感染者(包括48例慢性乙型肝炎、42例乙型肝炎肝硬化和78例乙型肝炎后肝癌患者)以及100例对照(感染后自发恢复者)进行HLA-DQA1等位基因的检测。结果:慢性HBV感染者HLA-DQA1*0102的表型频率显著低于对照组(25.6%vs47.0%,OR=0.39,Pc=0.003),DQA1*0601表型频率高于对照组(4.2%vs0,OR=0.96,P=0.039),但后者差异无统计学意义(Pc>0.05)。肝硬化患者DQA1*0104的表型频率显著低于无肝硬化患者(6.4%vs28.4%,OR=0.17,Pc=0.001),DQA1*0201的表型频率高于无肝硬化患者(27.7%vs12.2%,OR=2.76,P=0.014),但后者差异无统计学意义(Pc>0.05)。HLA-DQA1各等位基因的表型频率在肝癌患者与非肝癌患者间差异无统计学意义。结论:HLA-DQA1*0102等位基因可能降低慢性HBV感染的风险,而DQA1*0104等位基因可能降低乙型肝炎肝硬化的风险。乙型肝炎后肝癌的发生与HLA-DQA1等位基因无明显相关性。Objective： To investigate the association of HLA-DQA1 allele, chronic HBV infection, liver cirrhosis （LC）, and hepatocellular carcinoma （HCC）. Methods： The patients of 168 chronic HBV infection （including 48 chronic hepatitis B, 42 LC and 78 HCC patients） and 100 controls who had recovered from HBV infection were investigated for HLA-DQA1 typing by PCR/SSP technique, Results：In chronic HBV infection patients, the phenotype frequency of DQA1＊0102 was significantly decreased （25.6% vs 47.0%, OR = 0.39, Pc=0.003）, while DQA1＊0601 was significantly increased （4.2% vs 0.0%, OR=0.96, P=0.039） compared with controls. The frequency of DQA1＊0104 was significantly decreased （6.4% vs 28.4%, OR=0.17, Pc=0.001）, while, DQA1＊0201 was significantly increased （27.7% vs 12.2%, P = 0.014, OR = 2.76） in chronic HBV infection patients with LC compared to those without LC. But the differences of DQA1＊0601 and DQA1＊0201 failed to be significant after Bonferroni correction （Pc〉0.05）. There was no significant difference in DQA1 alleles between chronic HBV infection patients with or without HCC （P〉0.05）. Conclusion：HLA-DQA1＊0102 and DQA1＊0104 were associated with protection from chronic H BV infection and development of LC, respectively. There was no significant association between HLA-DQA1 and development of HCC in chronic HBV infection patients.

关 键 词：肝炎 乙型·肝肿瘤·HLA抗原 

分 类 号：R575[医药卫生—消化系统]