危重病人预警蛋白质组指纹表达与无效化疗的相关性研究  

Correlations between the LGT Proteomic Fingerprints and Ineffective Chemotherapy

作　　者：王蓉[1] 裴毅[2] 

机构地区：[1]山西医科大学第二临床医学院,太原030001 [2]山西省肿瘤医院老年病科,太原030013

出　　处：《现代生物医学进展》2007年第12期1858-1860,共3页Progress in Modern Biomedicine

基　　金：山西省科技攻关项目(2006031090-01)

摘　　要：目的:探索无效化疗与危重病人预警蛋白质组(LGT)指纹的相关性。方法:取昆明小鼠30只,平均体重19-22g,建立小鼠S180肉瘤动物模型,随机分为A(n=10)、B(n=5)、C(n=10)、D(n=5)四组,A组腹腔注射顺铂2.6mg/kg/d×4天,C组腹腔注射顺铂2.6mg/kg/d×8天,于用药结束后4天处死小鼠,B组和D组分别为肿瘤对照组(B组与A组处死小鼠时间相同,D组与C组处死时间相同,同时摘眼球取血进行SELDI(表面增强飞行时间质谱)技术检测,并计算瘤重。比较A组与B组、C组与D组之间肿瘤瘤重有无差异,并根据化疗后肿瘤与对照组比有无缩小分成化疗有效和无效组,比较二组之间LGT蛋白质组指纹出现率有无差异。结果:①模型鼠肿瘤瘤重与LGT蛋白质组指纹的出现率呈正相关,相关系数rs=1.00,P<0.01。A组瘤重与B组相比无统计学差异(P>0.05),C组瘤重明显高于D组,有统计学差异(P<0.05)。②各组LGT蛋白质组指纹的出现率分别为:A组:55.6% B组:50% C组:22.2% D组:80%。A组的LGT蛋白质组指纹出现率高于C组,相比有统计学差异(P<0.05)。结论:无效化疗可造成模型鼠肿瘤的增大和LGT蛋白质组指纹出现率的增高,表明肿瘤增大是造成肿瘤模型小鼠死亡的重要原因。Objective： To investigate the correlations between the lost goodwill target （LGT） proteomic fingerprints and ineffective chemotherapy, Methods： A mouse model was developed by inoculating S 180 cells directly into KM mice, All the mice were divided into 4 groups, Group A and C were treated with cisplatin with the dose of 2,6 mg/kg/d for 4 and 8 days by intraperitoneal injection, re- spectively. The mice were killed at the fourth day after chemotherapy. Group B and D were control groups. Mice in group A and B were killed at the same tine, and so did group C and D. Then the eyeball blood samples were collected and detected by SELDI （SEL- DI-TOF-MS： surface enhanced laser desorption / ionization time of flight mass spectrometry）. The tumor weights were calculated to compare the effectiveness of chemotherapy and the occurrences of LGT fingerprint were compared. Results： （i）The increment of mice tumor weight was related with the occurrence rate of LGT protein fingerprint, and the related coefficient （rs） was 1.00 （P〈0.01）. There was no significant difference in group A and group B （P〉0.05）, but group C was significantly different from group D （P〈0.05）. （2） The occurrence of LGT protein fingerprint of group A, B, C and D was 55.6%, 50%, 22.2%, 80%, respectively. Group A was significantly different from group C （P〈0.05）. Conclusions： The ineffective chemotherapy may lead to the increment of tumor weight and the high occurrence rate of LGT protein fingerprint. That is to say that the increment in tumor weight is an important reason of the death of model mice. LGT proteomic fingerprint may predict the tumor patient condition and the occurance of death.

关 键 词：动物模型 化疗 SELDI技术 

分 类 号：R730.231[医药卫生—肿瘤]