两株甲基叔丁基醚(MTBE)降解菌的驯化分离及降解试验  

Isolating of Two Pure Bacterial Cultures for Degradation of Methyl Tert-Butyl Ether (MTBE)

作　　者：胡勤海[1] 毛柯辉[1] 陈艳[1] 熊云龙[1] 朱妙军[1] 张杏青[1] 

机构地区：[1]浙江大学环境工程系,浙江杭州310029

出　　处：《农业环境科学学报》2007年第6期2085-2089,共5页Journal of Agro-Environment Science

摘　　要：从生长银杏树的土壤中驯化分离得到两株能降解MTBE的纯培养微生物,其中一株为球菌(BC-1),另一株为杆菌(BC-2),对这两菌株进行了室内培养降解试验。结果表明,两株纯培养微生物均能在好氧和一定MTBE浓度条件下,利用MTBE作为惟一的基质碳源生长;培养6d后,均能降解溶液中绝大部分的MTBE(浓度在5～81mg·L-1之间),降解率达99%以上;BC-2的降解速度快于BC-1,短期(<4d)降解效果也优于BC-1;高浓度的MTBE能抑制菌体的生长,甚至导致菌体细胞的死亡,BC-2对MTBE的耐受性优于BC-1;试验中检测到的MTBE降解产物有TBA、丙酮、TBF等,这些降解产物也能被微生物继续降解。Two pure bacterial cultures for degradation of methyl t-butyl ether （MTBE） were isolated from the soil under an old Gingko tree, and were named coccus BC-1 and bacillus BC-2 separately. Both of the two cultures can use MTBE as the sole carbon source to grow under aerobic condition in some concentration extension. After 6 days of incubation at 26-28 ℃, more than 99% of the MTBE （5-81 mg·L^-1） were degraded by BC-1 or BC-2 in the aqueous solution. The biodegradation rate of BC-2 was obviously faster than that of BC-1, as well as the effect of short-term （less than 4d） degradation. The results also showed that high concentration of MTBE would be an inhibitor and resulted in the death of cells. Compared with BC-1, BC-2 had better tolerability to the MTBE. Some degradation products were detected, including TBA, TBF and acetone. And these medium products could also be degraded by the two bacterial cultures discussed above.

关 键 词：MTBE 降解 微生物 驯化 

分 类 号：X172[环境科学与工程—环境科学]