大鼠β_2m^-/Thy-1^+骨髓源性肝干细胞的体外分选及鉴定  

Isolation and characterization of β_2m^-/Thy-1^+ bone marrow-derived liver stem cells from cholestatic rats in vitro

作　　者：兰玲[1] 孙超[1] 陈源文[1] 李定国[1] 

机构地区：[1]上海交通大学医学院新华医院消化内科,上海200092

出　　处：《上海交通大学学报（医学版）》2007年第11期1293-1296,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30500236)~~

摘　　要：目的尝试从骨髓干细胞(BMSC)中分选出具备干细胞和肝细胞双重特性的β2m-/Thy-1+骨髓源性肝干细胞(BDLSC)亚群,为细胞移植治疗肝病提供合适的供体细胞。方法利用免疫磁珠细胞分选(MACS)方法体外分选淤胆型大鼠模型的β2m-/Thy-1+BDLSC,流式细胞仪检测其纯度,RT-PCR法和免疫荧光染色法鉴定其肝相关性表型的表达。结果MACS法能成功分选纯化BDLSC,其在基因和蛋白水平均表达肝细胞样表型。结论β2m-/Thy-1+BDLSC是BMSC中较特异的肝干细胞亚群,具有可能益于肝病治疗的巨大潜力。Objective To explore the in vitro isolation of β2 m ^-/Thy-1^＋ bone marrow-derived liver stem cells （ BDLSCs ） which bear double features of stem and liver cells from bone marrow stem cells（BMSCs） as so to provide suitable donor cells for the treatment of liver diseases by cellular transplant. Methods β2m^-/Thy-1^＋ BDLSCs were isolated by magnetic bead cell sorting （MACS） method from cholestatic rats in vitro, and cell purity was detected using flow cytometry. Liver associated phenotype markers were characterized by RTPCR and immunofluorescence staining. Results BDLSCs isolated by MACS were purified and viable, and possessed hepatocyte-like features at gene and protein levels. Conclusion β2m^-/Thy-1^＋ BDLSCs are special subsets of BMSCs which may have promising potentials in the stem cell-based treatment of liver diseases.

关 键 词：骨髓干细胞 肝干细胞 磁珠 分选 

分 类 号：Q813[生物学—生物工程] R-332[医药卫生]