多孔β-磷酸三钙/胶原支架与犬牙周膜细胞三维复合体的构建  

Construction of 3D complex of porous β-tricalcium phosphate/collagen scaffolds and dog periodontal ligament cells

作　　者：钟金晟[1] 欧阳翔英[1] 梅芳[2] 邓旭亮[3] 曹采方[1] 

机构地区：[1]北京大学.口腔医学院牙周科,北京100081 [2]北京大学基础医学院解剖学与组织胚胎学系 [3]北京大学.口腔医学院特诊科,北京100081

出　　处：《北京大学学报（医学版）》2007年第5期507-510,共4页Journal of Peking University:Health Sciences

基　　金：国家863计划基金资助项目(2003AA302220)~~

摘　　要：目的:探索用多孔β-磷酸三钙/胶原(β-TCP/col)支架与犬牙周膜细胞(PDLCs)在体外构建三维复合体。方法:分离培养犬PDLCs,并对其来源进行鉴定;利用甲基噻唑基四唑法检测β-TCP/col浸提液对PDLCs增殖的影响;并将第3代PDLCs以2×105/mL的浓度接种于β-TCP/col支架上进行三维复合体的体外构建,用扫描电镜观察。结果:β-TCP/col浸提液对犬PDLCs增殖无不良影响;PDLCs可在材料的三维结构上贴附生长,细胞充分伸展,且表面可见明显分泌物。结论:多孔β-磷酸三钙/胶原支架与犬牙周膜细胞具有良好的生物相容性,二者可在体外构建成三维复合体,提示该材料具有成为牙周组织工程理想支架材料的潜力。Objective：To investigate the construction of 3D complex of porous β-tricalcium phosphate/collagen scaffolds（β-TCP/col）and dog periodontal ligament cells（PDLCs）.Methods：Dog PDLCs were isolated,cultured and identified.The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide（MTT）assay was used to evaluate the cytotoxicity of β-TCP/col on the proliferation of PDLCs.The cells were seeded onto porous β-TCP/col scaffolds.The cellular capability of adhesion and growth on porous β-TCP/col surface was investigated visually by scanning electron microscopy（SEM）.Results：The cytotoxicity assay indicated that there was no significant difference between β-TCP/col and the control during the 7 days（P〉0.05）.SEM showed cells successfully adhered to porous β-TCP/col scaffolds and spread extensively.Matrix secretions were found on the cell surface.Conclusion：Porous β-tricalcium phosphate/collagen scaffolds were of good biocompatibility to the dog periodontal ligament cells,and were potential ideal candidates for periodontal tissue engineering.

关 键 词：胶原 磷酸钙类 牙周膜 支架 组织工程性 

分 类 号：R783.1[医药卫生—口腔医学]