活性红M-3BE脱色真菌的筛选及脱色性能研究  

Screening of decolorizing fungi and decolorizing research for active red M-3BE

作　　者：张昊[1] 杨清香[1] 李慧君[1] 陈建军[1] 李学梅[1] 

机构地区：[1]河南师范大学生命科学学院,新乡453007

出　　处：《环境工程学报》2007年第11期55-58,共4页Chinese Journal of Environmental Engineering

基　　金：教育部"新世纪优秀人才支持计划"资助项目(NCET-05-0612);河南师范大学硕士科研启动基金资助项目(052052)

摘　　要：从自然环境中分离到2株对染料活性红M-3BE具有明显脱色效果的真菌，经形态学和26SrDNA序列分析，将其鉴定为Fusarium oxysporum和Geosmithia viridis。采用初始浓度50mg／LM-3BE的液体培养基同步脱色培养，F．oxysporum在24h内对M-3BE的脱色率为96％，G．viridis在36h内的脱色率为82％。对脱色酶系的检测结果表明，脱色过程中F．oxysporum能产生LiP和MnP，G．viridis则仅产生LiP。此外，F．oxysporu和G．viridis对另外7种染料的脱色率也可分别达到16％～100％和83．3％～100％。2 strains of fungi, with high decolorizing ability of active red M-3BE were isolated from the environment. According to the morphological characteristics and sequence analysis of 26S rDNA, the strains were identified as Fusarium oxysporum and Geosmithia viridis. The decolorization rate of 50 mg/L M-3BE by F. oxysporum could reach 96% within 24 h, and that of G. viridis＇s was 82% within 36 h. Detection results of decolorizing enzyme system indicated that F. oxysporum could produce two enzymes, Lip and MnP, however G. viridis only one enzyme, LiP. Extensive decolorization by F. oxysporu （ 16% - 100% ） and G. viridis （83. 3% 100% ） was obtained with other seven dyes.

关 键 词：活性红M-3BE 真菌 染料脱色 锰依赖过氧化物酶 木质素过氧化物酶 

分 类 号：X172[环境科学与工程—环境科学]