马铃薯卷叶病毒CP基因的RT-PCR扩增  

RT-PCR Amplification of PLRV Coat Protein Gene

作　　者：何心凤[1] 郭宝太[1] 李广存[2] 杨煜[2] 王晓杰[1] 毕玉平[2] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109 [2]山东省农业科学院高新技术中心,山东济南250100

出　　处：《中国马铃薯》2007年第4期197-199,共3页Chinese Potato Journal

基　　金：青岛市自然基金(05-1-JC-91)

摘　　要：根据马铃薯卷叶病毒CP基因的序列设计合成了两对特异性DNA引物,用RNA提取试剂RNAplant从感病的马铃薯叶片中提取总RNA,在3′引物引导下以总RNA为模板反转录合成cDNA第一链,然后进行PCR,分别扩增出了长627 bp和336 bp的特异性PCR产物,其大小与预期的CP基因及其部分序列一致,实现了马铃薯卷叶病毒CP基因及其片段的简便、有效的RT-PCR扩增。Two pairs of DNA primers were designed and synthesized according to the previously reported nucleotide sequence of potato leafroli virus （PLRV） coat protein（CP） gene. Total RNA was directly extracted from virus-infected potato leaves with RNAplant reagent and used as template for first chain synthesis of eDNA. Two specific PCR fragments about 630 bp and 330 bp were respectively obtained by reverse transcription polymerase chain reaction （RT-PCR） amplification, corresponding to the expected length of PLRV-CP gene and its partial sequence. Due to the application of RNA extraction reagent RNAplant, simple and effective RT-PCR amplification of PLRV-CP gene and its partial sequence was realized.

关 键 词：马铃薯 卷叶病毒 CP基因 RT-PCR 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]